A highly sensitive NADH biosensor using nitrogen doped graphene modified electrodes
IR@CECRI: CSIR-Central Electrochemical Research Institute, Karaikudi
View Archive Info| Field | Value | |
| Title |
A highly sensitive NADH biosensor using nitrogen doped graphene modified electrodes
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| Creator |
Mutyala, Sankararao
Mathiyarasu, J |
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| Subject |
Electrodics and Electrocatalysis
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| Description |
The present work describes the fabrication of highly sensitive and selective β-nicotinamide adenine dinucleotide
(NADH) sensor using nitrogen doped graphene (NDG). NDG modified glassy carbon electrode (GCE) exhibits
strong and stable electrocatalytic response towards NADH oxidation. A substantial (253 mV) decrease in the
overpotential for NADH oxidation reaction (compared to GCE) is observed at NDG/GCE. NDG was prepared
using hydrothermal procedure and characterized using spectroscopy and microscopy techniques. Furthermore,
amperometric responses of NDG/GCE have high sensitivity towards NADH oxidation at lower oxidation potential
and minimization of surface contamination. The fabricated NADH biosensor exhibited enhanced and reproducible
sensitivity of 0.16 μA/μM (S/N = 3) with a response time of b3 s and the calculated limit of detection is
0.37 μM. A linear dynamic range from0.5–12 μMwas observed for the fabricated biosensorwith a correlation coefficient
(R2) of 0.99. Moreover, the biosensor exhibited strong stability after prolonged usage. The oxidation
peak potential at the NDG/GCE remained unchanged even after 15 days, with minimum surface contamination.
Therefore, NDG materials are found useful and promising candidates for NADH detection and are attractive for
dehydrogenase based biosensor.
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| Publisher |
Elsevier B.V.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://cecri.csircentral.net/3164/1/091-2016.pdf
Mutyala, Sankararao and Mathiyarasu, J (2016) A highly sensitive NADH biosensor using nitrogen doped graphene modified electrodes. Journal of Electroanalytical Chemistry, 775. pp. 329-336. ISSN 1572-6657 |
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| Relation |
http://www.elsevier.com/locate/jelechem
http://cecri.csircentral.net/3164/ |
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