A benzimidazole-based chemodosimeter for the fluorometric detection of Zn and Cu via 1,5 proton shifts and C–N bond cleavage
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
A benzimidazole-based chemodosimeter for the
fluorometric detection of Zn and Cu via 1,5 proton
shifts and C–N bond cleavage
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| Creator |
Basu Roy, Sohini
Prodhan, Chandraday Chaudhuri, Keya Rajak, Kajal Krishna |
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| Subject |
Molecular & Human Genetics
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| Description |
Here, we report the design and synthesis of the fluorescent probe APBHN, which was derived from 2-
(1H-benzo[d]imidazol-2-yl)benzenamine and is capable of detecting intracellular Zn and Cu ions in the
micromolar range. Single-crystal X-ray analysis revealed that the structure of the ligand comprises a fused
cyclic system with a pendent naphthol moiety. With the addition of Zn and Cu ions the inherent fluorescence
behaviour of the ligand APBHN is perturbed via a chemodosimetric change that involves a 1,5
proton shift followed by C–N bond cleavage. Upon detailed analysis, it was found that the ligand forms
1 : 1 and 1 : 2 (metal to ligand) complexes with the corresponding metal ions. The detection limits of Zn2+
and Cu2+ were 5.59 μM and 0.148 μM, respectively, with APBHN, which are lower than the WHO guidelines
(76 μM for Zn2+ and 31.5 μM for Cu2+) for drinking water. Moreover, APBHN could be used as a practical,
visible colorimetric test kit for both Zn2+ and Cu2+. APBHN can efficiently detect Zn2+ and Cu2+ in
liver carcinoma cells with insignificant cytotoxicity
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| Date |
2017-07
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2714/1/Photochemical_%26_Photobiological_Sciences.pdf
Basu Roy, Sohini and Prodhan, Chandraday and Chaudhuri, Keya and Rajak, Kajal Krishna (2017) A benzimidazole-based chemodosimeter for the fluorometric detection of Zn and Cu via 1,5 proton shifts and C–N bond cleavage. Photochemical & Photobiological Sciences, 16 (7). pp. 1103-1116. |
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| Relation |
http://dx.doi.org/10.1039/c7pp00059f
http://www.eprints.iicb.res.in/2714/ |
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