Physical chemistry in a single live cell: confocal microscopy
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Physical chemistry in a single live cell:
confocal microscopy
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| Creator |
Amin, Md. Asif
Nandi, Somen Mondal, Prasenjit Mahata, Tanushree Ghosh, Surajit Bhattacharyya, Kankan |
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| Subject |
Chemistry
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| Description |
A live cell is a complex, yet extremely important container. Understanding the dynamics in a selected
intracellular component is a challenging task. We have recently made significant progress in this
direction using a confocal microscope as a tool. The smallest size of the focused spot in a confocal
microscope is B0.2 mm (200 nm). This is nearly one hundred times smaller than the size of a live cell.
Thus, one can selectively study different intracellular components/organelles in a live cell. In this paper,
we discuss how one can image different intracellular components/organelles, record fluorescence
spectra and decay at different locations, ascertain local polarity and viscosity, and monitor the dynamics
of solvation, proton transfer, red-ox and other phenomena at specified locations/organelles inside a cell.
We will highlight how this knowledge enriched us in differentiating between cancer and non-cancer
cells, 3D tumor spheroids and towards drug delivery
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| Date |
2017-05-28
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2717/1/Phys._Chem._Chem._Phys.%2C_2017%2C_19%2C_12620%2D%2D12627.pdf
Amin, Md. Asif and Nandi, Somen and Mondal, Prasenjit and Mahata, Tanushree and Ghosh, Surajit and Bhattacharyya, Kankan (2017) Physical chemistry in a single live cell: confocal microscopy. Phys. Chem. Chem. Phys, 19 (20). pp. 12620-12627. |
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| Relation |
http://dx.doi.org/10.1039/c7cp02228j
http://www.eprints.iicb.res.in/2717/ |
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