Protoplast formation and regeneration of Acetobacter pasteurianum NCIM 2314
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
Protoplast formation and regeneration of Acetobacter pasteurianum NCIM 2314
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| Creator |
Singhvi, Mamta
Joshi, Dipti Gokhale, D V |
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| Description |
94-96
Method for production and regeneration of <i>Acetobacter pasteurianum </i>NCIM 2314 protoplasts are described. The protoplasts were obtained by treatment with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplasts formation was obtained in protoplasts buffer with NaC1 as an osmotic stabilizer using lysozyme (0.5mg/mL). Maximum protoplast regeneation was obtained on Acetobacter medium with NaCl (0.5M) as an osmotic stabilizer. The regeneration medium was also applicable to other species of Acetobactor as well. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of <i>Acetobacter pasteurianum.</i> |
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| Date |
2011-08-16T07:03:46Z
2011-08-16T07:03:46Z 2011-06 |
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| Type |
Article
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| Identifier |
0975-2412 (Online); 0771-7706 (Print)
http://hdl.handle.net/123456789/12584 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
BVAAP Vol.19(1) [June 2011]
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