Characterization and <i style="">in vitro</i> expression of non-structural 1 protein of canine parvovirus (CPV-2) in mammalian cell line
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View Archive Info| Field | Value | |
| Title |
Characterization and <i style="">in vitro</i> expression of non-structural 1 protein of canine parvovirus (CPV-2) in mammalian cell line
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| Creator |
Saxena, Lovleen
Chaturvedi, Uttara Saxena, Shikha Kumar, G Ravi Sahoo, A P Kumar, Sudesh Doley, J Rajmani, R S Singh, Prafull K Kumar, Rajiv Tiwari, Ashok K |
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| Subject |
Eukaryotic expression vector
Canine parvovirus NS-1 gene Non-structural protein 1 Polymerase chain reaction |
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| Description |
654-659
Parvoviruses are small, 260-Å-diameter, icosahedral, non-enveloped, single-stranded DNA viruses with a genome of approximately 5 kb. Non structural protein, (NS-1) is especially relevant, being both essential for virus replication and the main factor responsible for virus pathogenicity and cytotoxicity. This protein has also been reported to possess the property of killing of transformed cells. The present study was carried out to clone, characterize and express the NS-1 gene of canine parvovirus. NS-1 complete CDS 2020bp was amplified, cloned into eukaryotic expression vector pcDNA 3.1(+), sequenced and characterized by <i style="">in vitro</i> expression analysis. Functional activity of recombinant construct, pcDNA.cpv.NS-1, was evaluated by RT-PCR and flow cytometry for the expression of NS-1 specific mRNA and NS-1 protein, respectively, in transfected HeLa cells. This recombinant plasmid may serve as an important tool to evaluate the apoptotic potential of NS-1 protein of canine parvovirus in cultured HeLa cells. |
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| Date |
2011-08-29T05:56:31Z
2011-08-29T05:56:31Z 2011-09 |
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| Type |
Article
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| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/12608 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJEB Vol.49(09) [September 2011]
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