Optimization of medium composition for the production of mosquitocidal toxins from <i style="mso-bidi-font-style:normal">Bacillus thuringiensis</i> subsp. <i style="mso-bidi-font-style:normal">israelensis</i>
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
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| Title |
Optimization of medium composition for the production of mosquitocidal toxins from <i style="mso-bidi-font-style:normal">Bacillus thuringiensis</i> subsp. <i style="mso-bidi-font-style:normal">israelensis</i>
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| Creator |
Poopathi, Subbiah
Archana, B |
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| Subject |
<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:9.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:Batang;letter-spacing:-.2pt;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Bacillus thuringiensis </span></i><span style="font-size:11.0pt;mso-bidi-font-size:9.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:Batang;letter-spacing:-.2pt;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">subsp. <i style="mso-bidi-font-style: normal">israelensis</i></span>
Coconut cake Cost-effectiveness <span style="font-size:11.0pt;mso-bidi-font-size: 9.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:Batang; letter-spacing:-.2pt;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:AR-SA" lang="EN-GB">MnCl<sub>2</sub></span> Mosquito larvae Toxicity assays |
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| Description |
65-71
Optimization of chicken feather (CF) based culture medium for the production of <i style="mso-bidi-font-style:normal">Bacillus thuringiensis</i> subsp. <i style="mso-bidi-font-style:normal">israelensis</i> (<i style="mso-bidi-font-style: normal">Bti</i>) biomass in combination with the agro industrial by-product (coconut cake, CC) and manganese chloride (MnCl<sub>2</sub>) has been evaluated. The biomass yield of <i style="mso-bidi-font-style: normal">Bti </i>spore/crystal toxin was highest <span style="color:black;mso-ansi-language:ES-CR" lang="ES-CR">(12.06 g/L) from the test medium (CF+CC+MnCl<sub>2</sub><span style="color:black; mso-ansi-language:ES-CR" lang="ES-CR">) compared to the reference medium (Luria Bertani, LB). Toxicity assay with <i style="mso-bidi-font-style:normal">Bti</i> produced from the test medium against mosquito vectors (<i style="mso-bidi-font-style:normal"><span style="mso-bidi-font-weight:bold">Culex quinquefasciatus</span></i><span style="mso-bidi-font-weight:bold">, <i style="mso-bidi-font-style:normal">Anopheles stephensi</i> and <i style="mso-bidi-font-style:normal">Aedes aegypti</i>) was also satisfactory and results were comparable with bacteria produced from LB. The results suggest that <i style="mso-bidi-font-style:normal">Bti</i> can be produced to the maximum extent possible as a potential mosquitocidal activity as suggested by the test medium (<span style="color:black; mso-ansi-language:ES-CR" lang="ES-CR">CF+CC+MnCl<sub>2</sub>)<span style="mso-bidi-font-weight:bold">. </span></span></span></span></span> |
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| Date |
2012-01-02T05:59:46Z
2012-01-02T05:59:46Z 2012-01 |
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| Type |
Article
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| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/13313 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJEB Vol.50(01) [January 2012]
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