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Functionalized polyacrylonitrile-nanofiber based immunosensor for Vibrio cholerae detection

IR@NPL: CSIR-National Physical Laboratory, New Delhi


Field	Value

Title	Functionalized polyacrylonitrile-nanofiber based immunosensor for Vibrio cholerae detection

Creator	Gupta, Pramod K. Gupta, A. Dhakate, S. R. Khan, Zishan H. Solanki, Pratima R.

Subject	Polymer Science

Description	Polyacrylonitrile nanofibers (PANnf's) were electrospun directly onto an indium tin oxide (ITO)-coated glass substrate. The PANnf/ITO electrode was partially hydrolyzed with an NaOH aqueous solution at ambient temperature to convert the nitrile groups of the PANnf's into carboxyl groups was confirmed by Fourier transform infrared spectroscopy. Furthermore, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxy succinimide chemistry was used to activate the ACOOH groups of PANnf's for the covalent co-immobilization of monoclonal antibodies against Vibrio cholerae and bovine serum albumin for V. cholerae toxin detection. Structural, functional, and electrochemical studies of the PANnf/ITO electrode and BSA/Ab/PANnf/ITO immunoelectrode were performed, and found a uniform distribution of nanofibers with diameter of 325+/-7.7% nm. The electrochemical response studies showed an improved sensing performance of the immunoelectrode with a detection of 6.25-500 ng/mL, a low limit of detection of 0.22 ng/mL, a sensitivity of 90 nA ng(-1) mL cm(-2), an association constant of 45.2 ng/mL, and a dissociation constant of 8 ng/mL.

Publisher	Wiley

Date	2016-11-20

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://npl.csircentral.net/2227/1/Functionalized%20polyacrylonitrile.pdf Gupta, Pramod K. and Gupta, A. and Dhakate, S. R. and Khan, Zishan H. and Solanki, Pratima R. (2016) Functionalized polyacrylonitrile-nanofiber based immunosensor for Vibrio cholerae detection. Journal of Applied Polymer Science, 133 (44). ISSN 0021-8995

Relation	http://npl.csircentral.net/2227/