Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB
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| Creator |
Upadhyay, Udita
Srivastava, Suchita Khatri, Indu Nanda, Jagpreet Singh Subramanian, Srikrishna Arora, Amit Singh, Jagmohan |
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| Subject |
QR Microbiology
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| Description |
Inactivation of retrotransposons is accompanied by the emergence of centromere-binding protein-B (CENPB) in Schizosaccharomyces, as well as in metazoans. The RNA interference (RNAi)-induced transcriptional silencing (RITS) complex, comprising chromodomain protein-1 (Chp1), Tas3 (protein with unknown function), and Argonaute (Ago1), plays an important role in RNAi-mediated heterochromatinization. We find that whereas the Ago1 subunit of the RITS complex is highly conserved, Tas3 is lost and Chp1 is truncated in Schizosaccharomyces cryophilus and Schizosaccharomyces octosporus We show that truncated Chp1 loses the property of heterochromatin localization and silencing when transformed in Schizosaccharomyces pombe Furthermore, multiple copies of CENPB, related to Tc1/mariner and Tc5 transposons, occur in all Schizosaccharomyces species, as well as in humans, but with loss of transposase function (except Schizosaccharomyces japonicus). We propose that acquisition of Tc1/mariner and Tc5 elements by horizontal transfer in S. pombe (and humans) is accompanied by alteration of their function from a transposase/endonuclease to a heterochromatin protein, designed to suppress transposon expression and recombination. The resulting redundancy of RITS may have eased the selection pressure, resulting in progressive loss or truncation of tas3 and chp1 genes in S. octosporus and S. cryophilus and triggered similar evolutionary dynamics in the metazoan orthologues.
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| Publisher |
The American Society for Microbiology
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| Date |
2017
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Article
PeerReviewed |
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| Relation |
http://dx.doi.org/10.1091/mbc.E16-07-0485
http://crdd.osdd.net/open/1997/ |
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| Identifier |
Upadhyay, Udita and Srivastava, Suchita and Khatri, Indu and Nanda, Jagpreet Singh and Subramanian, Srikrishna and Arora, Amit and Singh, Jagmohan (2017) Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB. Molecular Biology of the Cell, 28 (8). pp. 1132-1146. ISSN 1059-1524
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