CSIR Central

Saccharide binding by intelectins.

IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh


Field	Value

Title	Saccharide binding by intelectins.

Creator	Sharma, S Ramya, T N C

Subject	QR Microbiology

Description	This communication probes ligand binding by human Intelectin-1 with several saccharides. Human Intelectin-1 was previously reported to bind to microbial glycans via ribofuranoside or galactofuranoside residues, whereas subsequently, a crystal structure of ligand bound hITLN1 indicated that hITLN1 does not bind to ribofuranoside but distinguishes between microbial and human glycans through a glycan motif - a terminal, acyclic 1,2-diol, which is present on galactofuranose and other microbial saccharides. Here, we demonstrate that besides glycerol and glycerol derivatives (which have an acyclic 1,2-diol), and 2-deoxy-d-galactose, d-ribose and 2-deoxy-d-ribose, which have been previously reported as human Intelectin-1 ligands, 2-C-hydroxymethyl-d-ribose, d-talose, d-idose, d-altrose and sorbitol also elute human Intelectin-1 from Sepharose CL-6B. Interestingly, Sepharose, 2-deoxy-d-galactose (in its pyranose form), 2-C-hydroxymethyl-d-ribose, d-ribose and 2-deoxy d-ribose lack a terminal, acyclic 1,2-diol. We discuss the implications of these observations and rationalize the discrepancies in the apparent affinity of saccharide ligands for hITLN1 with different assay formats. We also report the distinct saccharide binding profiles of the hITLN1 homologues, HaloITLN and XL35ITLN, and demonstrate that hITLN1 binding to a saccharide ligand may modulate binding to its protein ligand, lactoferrin and vice versa.

Publisher	IPC Science and Technology Press

Date	2018-03

Type	Article PeerReviewed

Relation	https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)32687-9 http://crdd.osdd.net/open/2004/

Identifier	Sharma, S and Ramya, T N C (2018) Saccharide binding by intelectins. International journal of biological macromolecules, 108. pp. 1010-1016. ISSN 1879-0003