Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis
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| Creator |
Kumar, A
Karthikeyan, Subramanian |
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| Subject |
QR Microbiology
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| Description |
The MSMEG_4306 gene from Mycobacterium smegmatis encodes a protein of unknown function with 242 amino-acid residues that contains a conserved zinc-ribbon domain at its C-terminus. Here, the crystal structure of MSMEG_4306 determined by the single-wavelength anomalous dispersion method using just one zinc ion co-purified with the protein is reported. The crystal structure of MSMEG_4306 shows a coiled-coil helix domain in the N-terminal region and a zinc-ribbon domain in the C-terminal region. A structural similarity search against the Protein Data Bank using MSMEG_4306 as a query revealed two similar structures, namely CT398 from Chlamydia trachomatis and HP0958 from Helicobacter pylori, although they share only ∼15% sequence identity with MSMEG_4306. Based on comparative analysis, it is predicted that MSMEG_4306 may be involved in secretion systems, possibly by interacting with multiple proteins or nucleic acids.
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| Publisher |
John Wiley & Sons Inc
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| Date |
2018-03-01
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| Type |
Article
PeerReviewed |
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| Relation |
http://dx.doi.org/10.1107/S2053230X18002236
http://crdd.osdd.net/open/2016/ |
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| Identifier |
Kumar, A and Karthikeyan, Subramanian (2018) Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis. Acta Crystallographica Section F Structural Biology Communications, 74 (3). pp. 166-173. ISSN 2053-230X
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