An investigation into the taxonomy of “ Bacillus aminovorans ” and its reclassification to the genus Domibacillus as Domibacillus aminovorans sp. nov.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
An investigation into the taxonomy of “ Bacillus aminovorans ” and its reclassification to the genus Domibacillus as Domibacillus aminovorans sp. nov.
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| Creator |
Verma, Ashish
Ojha, Anup Kumar Pal, Yash Kumari, Parveen Schumann, Peter Gruber-Vodicka, Harald Dastager, Syed G. Natarajan, Ramesh Kumar Mayilraj, S. Krishnamurthi, Srinivasan |
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| Subject |
QR Microbiology
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| Description |
The species "Bacillus aminovorans" was categorized as species Incertae Sedis meaning taxa with uncertain taxonomic position because of insufficient description of phenotypic properties and lack of understanding of its phylogenetic relationship (Claus and Berkeley [5]). In this study two strains "B. aminovorans" DSM 1314T and DSM 4337 were phenotypically and phylogenomically analyzed. The strains are Gram-staining-positive, spore forming rods and unable to utilize/ferment most of the sugars tested except glucose. The major fatty acids are anteiso-C15:0 and iso-C15:0. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol and an aminophosphoglycolipid. The cell wall peptidoglycan is of A1γ type with meso-Dpm as the diamino acid. The menaquinone type present is MK-6. The G+C content of the genomic DNA of the type strain is 40.8mol%. These characteristics were found to be in line with description of the genus Domibacillus. Moreover phylogenetic analysis based on 16S rRNA gene sequence retrieved "B. aminovorans" within the genus Domibacillus with D. antri XD 80T as the closest relative. Additionally genome sequencing of the strains and their comparison with whole genome sequences of other Domibacillus spp. confirmed their distinctiveness and separate species status within the genus based on parameters of genome to genome distance calculator (GGDC) and average nucleotide identity (ANI) values. Therefore a novel species Domibacillus aminovorans sp. nov. (DSM 1314T=LMG 16796T) is proposed.
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| Publisher |
Elsevier
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| Date |
2017
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Article
PeerReviewed |
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| Relation |
http://dx.doi.org/10.1016/j.syapm.2017.07.003
http://crdd.osdd.net/open/2038/ |
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| Identifier |
Verma, Ashish and Ojha, Anup Kumar and Pal, Yash and Kumari, Parveen and Schumann, Peter and Gruber-Vodicka, Harald and Dastager, Syed G. and Natarajan, Ramesh Kumar and Mayilraj, S. and Krishnamurthi, Srinivasan (2017) An investigation into the taxonomy of “ Bacillus aminovorans ” and its reclassification to the genus Domibacillus as Domibacillus aminovorans sp. nov. Systematic and Applied Microbiology, 40 (7). pp. 458-467. ISSN 07232020
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