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Microbial F-type lectin domains with affinity for blood group antigens

IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh


Field	Value

Title	Microbial F-type lectin domains with affinity for blood group antigens

Creator	Mahajan, Sonal Khairnar, Aasawari Bishnoi, Ritika Ramya, T.N.C.

Subject	QR Microbiology

Description	F-type lectins are fucose binding lectins with characteristic fucose binding and calcium binding motifs. Although they occur with a selective distribution in viruses, prokaryotes and eukaryotes, most biochemical studies have focused on vertebrate F-type lectins. Recently, using sensitive bioinformatics search techniques on the non-redundant database, we had identified many microbial F-type lectin domains with diverse domain organizations. We report here the biochemical characterization of F-type lectin domains from Cyanobium sp. PCC 7001, Myxococcus hansupus and Leucothrix mucor. We demonstrate that while all these three microbial F-type lectin domains bind to the blood group H antigen epitope on fucosylated glycans, there are fine differences in their glycan binding specificity. Cyanobium sp. PCC 7001 F-type lectin domain binds exclusively to extended H type-2 motif, Myxococcus hansupus F-type lectin domain binds to B, H type-1 and Lewisb motifs, and Leucothrix mucor F-type lectin domain binds to a wide range of fucosylated glycans, including A, B, H and Lewis antigens. We believe that these microbial lectins will be useful additions to the glycobiologist's toolbox for labeling, isolating and visualizing glycans.

Publisher	Elsevier

Date	2017

Type	Article PeerReviewed

Relation	http://dx.doi.org/10.1016/j.bbrc.2017.07.125 http://crdd.osdd.net/open/2063/

Identifier	Mahajan, Sonal and Khairnar, Aasawari and Bishnoi, Ritika and Ramya, T.N.C. (2017) Microbial F-type lectin domains with affinity for blood group antigens. Biochemical and Biophysical Research Communications, 491 (3). pp. 708-713. ISSN 0006291X