Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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| Title |
Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor
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| Creator |
Singh, S.
Solanki, P. R. Pandey, M. K. Malhotra, B. D. |
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| Subject |
Analytical Chemistry
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| Description |
Cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) have been covalently immobilized on electrochemically prepared polyaniline (PANI) films. These PANI/ChEt/ChOx enzyme films have been characterized using UV-visible, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). Electrochemical behavior of these films has been studied using cyclic voltammetry (CV) and amperometric techniques, respectively. The PANI/ChEt/ChOx enzyme films show broad oxidation peak from 0.2 to 0.5 V. These PANI/ChEt/ChOx biosensing electrodes have a response time of about 40 s, linearity from 50 to 500 mg/dl of cholesterol oleate concentration. These PANI/ChEt/ChOx films are thermally stable up to 46 degrees C. This polyaniline based cholesterol biosensor has optimum pH in the range of 6.5-7.5, sensitivity as 7.5 x 10(-4) nA/mg dl and a lifetime of about 6 weeks.
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| Publisher |
Elsevier
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| Date |
2006-05-24
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/2439/1/26.pdf
Singh, S. and Solanki, P. R. and Pandey, M. K. and Malhotra, B. D. (2006) Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor. Analytica Chimica Acta , 568 (1-2). pp. 126-132. ISSN 0003-2670 |
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| Relation |
http://npl.csircentral.net/2439/
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