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Real-Time PCR assay and melting curve analysis for identification of diarrheagenic <em>Escherichia coli</em> in paediatric population

IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)


Field	Value

Title	Real-Time PCR assay and melting curve analysis for identification of diarrheagenic <em>Escherichia coli</em> in paediatric population

Creator	Singh, Taru Das, Shukla Ramachandran, VG Maroof, Khan Amir Rai, Arvind

Subject	Diarrhoea <em>E</em>.<em> coli</em> infections Real-Time PCR

Description	876-879 In developing countries, including India, diarrhea causes considerable death among infants and children. Early diagnosis plays a vital role in preventing the fatality rate. Conventional methods for early detection of diarrheagenic <em>E. coli</em> (DEC) are complicated and inaccurate. Hence, in this study we proposed a syber green based Real-Time PCR to determine the prevalence of DEC. Detection of diarrheagenic <em>E. coli </em> using Real-Time Syber Green based PCR assay was performed in 120 isolates including 80 isolates from healthy children. Virulent genes were detected in 106 (88.3%) samples [39 (97.5%) and 67 (83.7%)] in cases and controls, respectively. EPEC was found (<em>typical</em>, <em> atypical </em> and <em>bfp</em>) in 87 (72.5%) specimens followed by EAEC (<em>eagg</em>) in 76 (63.3%), ETEC (<em>elt </em> and e<em>st</em>) in 55 (45.8%) and EHEC (<em>stx</em> and <em>hyla</em>) in 11 (9.2%) specimens. Melting curve analysis showed distinct peaks for all target genes.

Date	2017-12-08T07:28:52Z 2017-12-08T07:28:52Z 2017-12

Type	Article

Identifier	0975-1009 (Online); 0019-5189 (Print) http://nopr.niscair.res.in/handle/123456789/43227

Language	en_US

Rights	<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>

Publisher	NISCAIR-CSIR, India

Source	IJEB Vol.55(12) [December 2017]