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Kinetic Study and Characterization of Cellulase Enzyme from Isolated <em>Aspergillus niger subsp. awamori</em> for Cellulosic Biofuels

IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)


Field	Value

Title	Kinetic Study and Characterization of Cellulase Enzyme from Isolated <em>Aspergillus niger subsp. awamori</em> for Cellulosic Biofuels

Creator	Pachauri, P More, S Aranganathan, V Sullia, S B Deshmukh, S

Subject	Cellulase <i>Aspergillus Niger</i> <i>Subsp. Awamori</i> Purification Lignocellulosic Biomass

Description	55-60 In the present study, a cellulase producing <em>Aspergillus </em> species was isolated from wood chips and was identified as <em>Aspergillus niger subsp. awamori</em> (also called <em>A. awamori</em>) using 18s rRNA sequencing (Genbank Accession number KP164968). The enzyme was purified to 42.5 fold by gel filtration chromatography and the molecular mass of the enzyme was determined as 66±1 kDa using SDS-PAGE. The purified enzyme showed an optimum temperature of 50°C and pH 5. Metal ions such as Ca<sup>2+</sup>, Mg<sup>2+</sup>, Zn<sup>2+</sup>, Pb<sup>2+</sup>, Fe<sup>2+</sup> and Fe<sup>3+ </sup> had positive influence on the enzyme activity. The K<sub>M </sub> and V<sub>max</sub> for the enzyme was found to be 0.011g and 0.1098U/ml respectively. The addition of this cellulase to lignocellulosic biomass showed good degradation of cellulose and therefore this fungus could be a future source for production of bioethanol from Lignocellulosic Biomass (LB).

Date	2018-01-03T10:02:34Z 2018-01-03T10:02:34Z 2018-01

Type	Article

Identifier	0975-1084 (Online); 0022-4456 (Print) http://nopr.niscair.res.in/handle/123456789/43308

Language	en_US

Rights	<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>

Publisher	NISCAIR-CSIR, India

Source	JSIR Vol.77(01) [January 2018]