FTIR mapping of peels of <em>Litchi chinensis</em> after acid treatment and its SSF for ethanol production by <em>Pachysolen tannophilus</em> MTCC 1077
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View Archive Info| Field | Value | |
| Title |
FTIR mapping of peels of <em>Litchi chinensis</em> after acid treatment and its SSF for ethanol production by <em>Pachysolen tannophilus</em> MTCC 1077
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| Creator |
Bhatia, Latika
Johri, Sonia |
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| Subject |
<em>Litchi chinensis</em> peel
Steam explosion FTIR Enzymatic hydrolysis RSM HPLC |
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| Description |
433-445
Pulverized peels of <em>L. chinensis</em> consists of 40 ± 0.38% cellulose, 28 ± 0.18% hemicellulose and 25 ± 0.31% of lignin on dry solid basis. About 1% HNO<sub>3</sub> delignified peels of <em>L. chinensis</em> yielded 46.71% xyloseand 12.42% arabinose under steam explosion, with a hydrolytic efficiency of 78.2 ± 0.45%. Fourier transform infrared (FTIR) spectroscopy results not only indicated the penetration of HNO<sub>3 </sub> in the amorphous region of the biomass and degradation of hemicelluloses but also revealed the structural differences before and after pretreatment. Simultaneous saccharification and fermentation (SSF) of pretreated peels of <em>L. chinensis</em> by crude cellulase and immobilized yeast <em>Pachysolen tannophilus</em> MTCC 1077 were investigated in the present study. Important process variables for ethanol production from pretreated peels of <em>L. chinensis</em> were optimized using response surface methodology (RSM) based on central composite design (CCD) experiments. A three level CCD experiments with central and axial points was used to develop a statistical model for the optimization of process variables such as incubation temperature (30°C, 32°C and 34°C) X1, inoculum level (2%, 4% and 6%) X2 and nutrients (1/2/3) X3. Data obtained from RSM on ethanol production were subjected to the analysis of variance (ANOVA) and analyzed using a second order polynomial equation and contour plots were used to study the interactions among three relevant variables of the fermentation process. The processing parameters setup for reaching a maximum response for ethanol production was obtained when applying the optimum values for temperature (30°C), inoculum level (6%) and fermentation medium (ammonium sulphate, KH<sub>2</sub>PO<sub>4</sub>, peptone and yeast extract) for <em>P. tannophilus</em> MTCC 1077. Maximum ethanol concentration 10.5 g/l was obtained after 72 h from <em>P. tannophilus</em> MTCC 1077 at the optimized process conditions in anaerobic batch fermentation. |
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| Date |
2018-01-04T04:32:26Z
2018-01-04T04:32:26Z 2017-07 |
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| Type |
Article
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| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/43325 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBT Vol.16(3) [July 2017]
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