Influence of growth conditions on enhanced production of alpha amylase from <em>Penicillium </em> species in solid state fermentation
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
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Title |
Influence of growth conditions on enhanced production of alpha amylase from <em>Penicillium </em> species in solid state fermentation
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Creator |
Abdullah, Roheena
Nadeem, Samia Iqtedar, Mehwish Kaleem, Afshan Iftikhar, Tehreema Naz, Shagufta |
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Subject |
Alpha amylase
<i>Penicilium</i> Growth conditions Solid state fermentation |
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Description |
426-432
The present study deals with the production of alpha amylase by <em>Penicillium</em> sp. employing solid state fermentation. Seven different media were evaluated. Out of all media tested wheat bran with distilled water gave maximum production of alpha amylase. Different parameters such as substrate concentration, moisture content, temperature, incubation time, size of inoculum and pH were optimized for the enzyme production. The maximum enzyme production was obtained by using 7.5 g of wheat bran, 100% of moisture content, incubation period 72 h, temperature of 30°C, inoculum size of 13.3% and pH 6. Addition of 1.5% starch as carbon source and 0.2% NH<sub>4</sub>NO<sub>3 </sub> and 2% yeast extract as inorganic and organic nitrogen sources respectively gave maximum production of alpha amylase. In addition to this effect of metal ions was also investigated CaCl<sub>2 </sub> act as stimulator for enzyme production by <em>Penicillium</em> sp. |
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Date |
2018-01-04T04:35:47Z
2018-01-04T04:35:47Z 2017-07 |
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Type |
Article
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Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/43326 |
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Language |
en_US
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Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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Publisher |
NISCAIR-CSIR, India
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Source |
IJBT Vol.16(3) [July 2017]
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