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Influence of growth conditions on enhanced production of alpha amylase from <em>Penicillium </em> species in solid state fermentation

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Title Influence of growth conditions on enhanced production of alpha amylase from <em>Penicillium </em> species in solid state fermentation
 
Creator Abdullah, Roheena
Nadeem, Samia
Iqtedar, Mehwish
Kaleem, Afshan
Iftikhar, Tehreema
Naz, Shagufta
 
Subject Alpha amylase
<i>Penicilium</i>
Growth conditions
Solid state fermentation
 
Description 426-432
The present study deals with the production of alpha amylase by <em>Penicillium</em> sp. employing solid state fermentation. Seven different media were evaluated. Out of all media tested wheat bran with distilled water gave maximum production of alpha amylase. Different parameters such as substrate concentration, moisture content, temperature, incubation time, size of inoculum and pH were optimized for the enzyme production. The maximum enzyme production was obtained by using 7.5 g of wheat bran, 100% of moisture content, incubation period 72 h, temperature of 30°C, inoculum size of 13.3% and pH 6. Addition of 1.5% starch as carbon source and 0.2% NH<sub>4</sub>NO<sub>3 </sub> and 2% yeast extract as inorganic and organic nitrogen sources respectively gave maximum production of alpha amylase. In addition to this effect of metal ions was also investigated CaCl<sub>2 </sub> act as stimulator for enzyme production by <em>Penicillium</em> sp.
 
Date 2018-01-04T04:35:47Z
2018-01-04T04:35:47Z
2017-07
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/43326
 
Language en_US
 
Rights <img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
 
Publisher NISCAIR-CSIR, India
 
Source IJBT Vol.16(3) [July 2017]