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Kinetics of enzymatic hydrolysis of Indian tuna fish oil using <em>Candida rugosa </em> lipase

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Field	Value

Title	Kinetics of enzymatic hydrolysis of Indian tuna fish oil using <em>Candida rugosa </em> lipase

Creator	Bhandari, Kriti Chaurasia, S P Singh, K

Subject	Indian tuna fish oil <em>Candida rugosa</em> lipase Hydrolysis Biphasic system

Description	413-418 Tuna fish oil is one of the richest natural source of docosahexaenoic acid (DHA) having nutraceutical and pharmaceutical potential. DHA has been also known as brain food and is also useful in prevention and control of various human diseases and disorders. The present paper investigates the kinetic study of Indian tuna fish oil catalyzed by <i>Candida rugosa lipase</i> (CRL) in biphasic solvent system. In 24 h, 80.1% hydrolysis was achieved. For hydrolysis, second order product inhibition was used for kinetic model fitting. The kinetic model was fitted using MATLAB® to determine the best kinetic parameters. The average value of kinetic constants were estimated as K<sub>M</sub> = 12 μ moles FFA/ml, K<sub>i1</sub> = 2.0 X 10<sup>-8</sup> μ moles free fatty acids (FFA)/mg enzyme•h, K<sub>i2</sub> = 0.078 μ moles free fatty acids (FFA)/mg enzyme•h and K<sub>2</sub> = 652.7 μ moles FFA/mg enzyme•h.R<sub>2</sub>, root mean square error (RMSE), mean absolute percentage error (MAPE) was found to be 0.959 and 60.51 and 11.41, respectively.

Date	2018-01-04T05:29:36Z 2018-01-04T05:29:36Z 2017-07

Type	Article

Identifier	0975-0967 (Online); 0972-5849 (Print) http://nopr.niscair.res.in/handle/123456789/43328

Language	en_US

Rights	<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>

Publisher	NISCAIR-CSIR, India

Source	IJBT Vol.16(3) [July 2017]