Stability studies of cuticle degrading and mycolytic enzymes of <em>Myrothecium verrucaria</em> for control of insect pests and fungal phytopathogens
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
Stability studies of cuticle degrading and mycolytic enzymes of <em>Myrothecium verrucaria</em> for control of insect pests and fungal phytopathogens
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| Creator |
Chavan, S B
Vidhate, R P Kallure, G S Dandawate, N L Khire, J M Deshpande, M V |
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| Subject |
CDE/ME complex
<i>Myrothecium verrucaria</i> <i>Helicoverpa armigera</i> Polyols <i>Sclerotium rolfsii</i> |
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| Description |
404-412
<em>Myrothecium verrucaria</em> produced extracellularly hydrolytic enzymes which can hydrolyse the insect cuticle as well as fungal cell wall. The addition of polyols, such as glycerol, sorbitol, xylitol (1 M) during ultra-filtration or freeze- drying of enzyme mixture increased the recovery of the enzymes in a concentrated form. Polyols (5 M) increased the temperature and pH stability of the enzymes, in the presence of glycerol chitinase , β-1,3-glucanase, lipase and protease retained 50-60% of initial activities at 40°C after 3 h. While xylitol (5 M) was effective in stabilizing activities at pH 5.0 and 7.5 at 25°C for 7 d. In the freeze-dried powder form, > 90% at 4°C for 1 year and 80-85% at 25°C for 2 months enzyme activities were retained. The addition of glycerol (1 M) to the enzyme mixture protected enzyme activities under sunlight (60-65% activity at RT) for 5 d. Because of glycerol (1 M), the efficacy of <em>M. verrucaria </em> enzyme preparation to control <em>Helicoverpa armigera</em> infestation in chick pea was increased to 70 ± 19%. While without glycerol the efficacy was 55 ± 23%. The germination of peanut seeds infected with <em>Sclerotium rolfsii</em> was observed to be increased (70 ± 5%) in a pot irrigated with enzyme mixture. |
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| Date |
2018-01-04T05:39:24Z
2018-01-04T05:39:24Z 2017-07 |
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| Type |
Article
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| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/43329 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBT Vol.16(3) [July 2017]
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