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Isolation and partial characterization of cellulase free alkalo-thermophilic xylanase produced by <em> Bacillus flexus</em>

IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)


Field	Value

Title	Isolation and partial characterization of cellulase free alkalo-thermophilic xylanase produced by <em> Bacillus flexus</em>

Creator	Bora, Sudipta Sankar Das, Saurav Lahan, Jyoti Prasad Barooah, Madhumita

Subject	<i>Bacillus flexus</i> Xylanase Alkalothermophilic

Description	396-403 <em>Bacillus flexus,</em> an alkalophilic bacterial strain, was isolated from tea rhizosphere of GolaghatDistrict of Assam by culture enrichment method. During the study the strain was found to produce cellulose-free xylanase of molecular weight 25 kDa which was determined using SDS-PAGE analysis. The enzyme had high hydrolytic activity against birchwoodxylan, oat spelt xylan and beech wood xylan; and exhibited relatively low activity against cellobiose, starch, carboxy methyl cellulose and avicel. Optimum PH and temperature in which enzyme was found active at pH 8.0 ± 0.2 and at 70°C ± 2°C respectively. The K<sub>m</sub> and V<sub>max</sub> values were found to be 0.9 mg/ml and 3633 U/ml, respectively. Hydrolytic activity of the enzyme with metal ions were also determined and interestingly it was found that Co<sup>2+</sup>, Zn<sup>2+</sup>, Mg<sup>2+</sup>, Fe<sup>3+</sup> and Ca<sup>2+ </sup> elevated the enzyme activity while Hg<sup>2+ </sup> and metal chelator EDTA reduced its activity.

Date	2018-01-04T06:02:51Z 2018-01-04T06:02:51Z 2017-07

Type	Article

Identifier	0975-0967 (Online); 0972-5849 (Print) http://nopr.niscair.res.in/handle/123456789/43330

Language	en_US

Rights	<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>

Publisher	NISCAIR-CSIR, India

Source	IJBT Vol.16(3) [July 2017]