<em>In vitro</em> propagation of <i>Nyctanthes arbor-tristis</i></em> L.
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
<em>In vitro</em> propagation of <i>Nyctanthes arbor-tristis</i></em> L.
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| Creator |
Raja, H David
Arockiasamy, D I |
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| Subject |
<em>In vitro</em> propagation
<em>Nyctanthes arbor-tristis</em> Plant regeneration Culture condition |
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| Description |
366-369
Mass propagation of tree species has always been a challenging task. An efficient protocol for rapid <em>in vitro</em> mass propagation of <em> Nyctanthes arbor-tristis </em> L<em>. </em> has been developed from shoot tip and internodal explants. Explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzylamino purine (BAP) and thidiazuron (TDZ) in various concentrations. 2. 0 mg/l BAP was proved to be best for high frequency multiple shoot induction from shoot-tip explants and 2. 5 mg/l BAP was produced maximum shoots proliferation from internodal explants. The well elongated healthy shoots were transferred for rooting on MS medium supplemented with indole-3-butyric acid (IBA) in different concentration. Maximum numbers of roots were achieved in 2.0 mg/l of IBA medium. The rooted plantlets were hardened and transferred to field conditions. All the regenerated plants with 80-85% survival rate were normal with respect to morphology and growth characteristics. |
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| Date |
2018-01-04T06:17:17Z
2018-01-04T06:17:17Z 2017-07 |
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| Type |
Article
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| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/43334 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBT Vol.16(3) [July 2017]
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