Roseovarius azorensis sp. nov., isolated from seawater at Espalamaca, Azores
IR@NIO: CSIR-National Institute Of Oceanography, Goa
View Archive Info| Field | Value | |
| Creator |
Rajasabapathy, R.
Mohandass, C. Dastager, S.G. Liu, Q. Khieu, T.-N. Son, C.K. Li, W.-J. Colaco, A. |
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| Date |
2014-03-05T10:44:24Z
2014-03-05T10:44:24Z 2014 |
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| Identifier |
Antonie van Leeuwenhoek, vol.105(3); 2014; 571-578
http://drs.nio.org/drs/handle/2264/4467 |
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| Description |
A Gram-negative, motile, non-spore forming, rod shaped aerobic bacterium, designated strain SSW084<sup>T</sup>, was isolated from a surface seawater sample collected at Espalamaca (38°33`N; 28°39`W), Azores. Growth was found to occur from 15 to 40 °C (optimum 30 °C), at pH 7.0–9.0 (optimum pH 7.0) and with 25–100 % seawater or 0.5–7.0 % NaCl in the presence of Mg2<sup>+</sup> and Ca2<sup>+</sup>; no growth was found with NaCl alone. Colonies on seawater nutrient agar were observed to be punctiform, white, convex, circular, smooth, and translucent. Strain SSW084<sup>T</sup> did not grow on Zobell marine agar and tryptic soy agar even when seawater supplemented. The major respiratory quinone was found to be Q-10 and the G + C content was determined to be 61.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SSW084<sup>T</sup> belongs to the genus Roseovarius and that its closest neighbours are Roseovarius tolerans EL-172<sup>T</sup>), Roseovarius mucosus DFL-24<sup>T</sup> and Roseovarius lutimaris 112<sup>T</sup> with 95.7, 95.4 and 95.3 % sequence similarity respectively. The remaining species of Roseovarius showed <95 % similarity. The polar lipids of strain SSW084<sup>T</sup> were determined to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified lipid and one unidentified aminolipid. The major fatty acids identified were identified as C<sub>18:1</sub> ω7c (52.5 %) and C<sub>16:0</sub> (13.8 %). On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain SSW084<sup>T</sup> is considered to represent a novel species of the genus Roseovarius, for which Roseovarius azorensis sp. nov is proposed. The type strain is SSW084<sup>T</sup> (=KCTC 32421<sup>T</sup> = MTCC 11812<sup>T</sup>)
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| Language |
en
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| Publisher |
Springer
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| Rights |
An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2014] Springer.
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| Subject |
new species
biological speciation genetic isolation phylogenetics aerobic bacteria fatty acids |
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| Title |
Roseovarius azorensis sp. nov., isolated from seawater at Espalamaca, Azores
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| Type |
Journal Article
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