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Studies on antimicrobial activity and physicochemical properties of the chitin and chitosan isolated from shrimp shell waste

IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)

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Title Studies on antimicrobial activity and physicochemical properties of the chitin and chitosan isolated from shrimp shell waste
 
Creator Jadhav, Aniruddha B
Diwan, Arvind D
 
Subject Shrimp shells
Chitin
Chitosan
DDA
Antibacterial activity
 
Description 674-680
Chitin and chitosan were isolated from shrimp shells waste by various chemical treatments. 1N HCl at ambient temperature was used for demineralization step and 0.5M NaOH solution at 90°C for 20 hours was used for deproteinization step. The obtained chitin was subsequently treated with high concentrations of NaOH (70% - 85%) at various temperatures for different time periods to achieve deacetylation. Various physicochemical properties of isolated chitin and chitosan were evaluated and found to be matching with commercially available chitin and chitosan. The NaOH concentration and temperature were affecting the degree of deacetylation (DDA).  Antibacterial profile of isolated chitosan was checked against various potential human pathogens and food contaminants by disc diffusion method. The DDA greatly influences antibacterial activity probably due to free NH<sub>2</sub><sup>+</sup> groups. The chitosan with highest DDA was found to be having high antibacterial activity against all strains.
 
Date 2018-03-27T04:57:51Z
2018-03-27T04:57:51Z
2018-03
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/handle/123456789/44114
 
Language en_US
 
Rights <img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.47(03) [March 2018]