Studies on antimicrobial activity and physicochemical properties of the chitin and chitosan isolated from shrimp shell waste
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
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Title |
Studies on antimicrobial activity and physicochemical properties of the chitin and chitosan isolated from shrimp shell waste
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Creator |
Jadhav, Aniruddha B
Diwan, Arvind D |
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Subject |
Shrimp shells
Chitin Chitosan DDA Antibacterial activity |
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Description |
674-680
Chitin and chitosan were isolated from shrimp shells waste by various chemical treatments. 1N HCl at ambient temperature was used for demineralization step and 0.5M NaOH solution at 90°C for 20 hours was used for deproteinization step. The obtained chitin was subsequently treated with high concentrations of NaOH (70% - 85%) at various temperatures for different time periods to achieve deacetylation. Various physicochemical properties of isolated chitin and chitosan were evaluated and found to be matching with commercially available chitin and chitosan. The NaOH concentration and temperature were affecting the degree of deacetylation (DDA). Antibacterial profile of isolated chitosan was checked against various potential human pathogens and food contaminants by disc diffusion method. The DDA greatly influences antibacterial activity probably due to free NH<sub>2</sub><sup>+</sup> groups. The chitosan with highest DDA was found to be having high antibacterial activity against all strains. |
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Date |
2018-03-27T04:57:51Z
2018-03-27T04:57:51Z 2018-03 |
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Type |
Article
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Identifier |
0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/handle/123456789/44114 |
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Language |
en_US
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Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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Publisher |
NISCAIR-CSIR, India
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Source |
IJMS Vol.47(03) [March 2018]
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