Isolation and purification of protease from <em>Labeo rohita</em> viscera
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
Isolation and purification of protease from <em>Labeo rohita</em> viscera
|
|
| Creator |
Geethanjali, S.
Subash, Anitha |
|
| Subject |
Acetone
Crude enzyme Digestive protease Fresh water fish Rohu Visceral protease |
|
| Description |
222-226
The discarding of fish wastes creates environmental and disposal problems. A number of bioactive compounds can be extracted and purified from the fish wastes. Therefore, protease was purified from the visceral organ wastes of <em>Labeo rohita.</em> The fish visceral waste was collected from Mettur Dam, Tamil Nadu. A crude homogenate was prepared and subjected to acetone precipitation, dialysis, Sephadex G-100 and DEAE cellulose column chromatography. The molecular weight of the isolated protease was determined by SDS-PAGE and confirmation of the protease activity was done by zymography. From the study, it was concluded that, the final purified sample showed a purification fold of 13.4, the specific activity of 8.62 U/mg, recovery percentage of 5.44 and a molecular weight of 38 kDa. Hence the present study was taken up with the view of utilizing the large amounts of fish wastes for the extraction of a beneficial enzyme, namely protease thereby contributing to the reduction of pollution caused by disposal of fish waste. |
|
| Date |
2018-06-27T10:01:19Z
2018-06-27T10:01:19Z 2018-06 |
|
| Type |
Article
|
|
| Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/44624 |
|
| Language |
en_US
|
|
| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
|
|
| Publisher |
NISCAIR-CSIR, India
|
|
| Source |
IJBB Vol.55(3) [June 2018]
|
|