New cell surface bound lectins with complex carbohydrate specificity from members of green algae
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
New cell surface bound lectins with complex carbohydrate specificity from members of green algae
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| Creator |
Singh, Ram S
Walia, Amandeep K Kaur, Jaspreet Singh, Davinder P Rajput, Alka |
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| Subject |
Agglutination
Carbohydrate specificity <i>Chlorella vulgaris</i> <i>Chlorococcum infusiformis</i> Chlorophyceae <i>Desmodesmus dimorphus</i> <i>Desmodesmus subspicatus</i> Lectin <i>Scenedesmus quadricauda</i> |
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| Description |
484-492
Lectins, algal in particular, have immense potential for biomedical applications such as anti-HIV, antitumoral, antimicrobial, anti-inflammatory and antinociceptive activities. In this context, there is a growing interest among researchers on agglutinins from green algae. Here, we have made an attempt to catalogue lectins from various unexplored green algae species. Chlorophyceae members (<em>Chlorella vulgaris</em>,<em> Chlorococcum infusiformis</em>,<em> Desmodesmus dimorphus</em>,<em> D. subspicatus </em> and <em>Scenedesmus quadricauda</em>) were screened for lectin activity using human, pig, sheep, goat and rabbit erythrocytes. All of them showed surface bound lectin activity with highest agglutination titre towards human blood type B erythrocytes and rabbit erythrocytes. Neuraminidase and protease treatment to human blood type B erythrocytes considerably enhanced the agglutination titre of lectins from <em>S. quadricauda</em>, <em>C. vulgaris</em> and <em>D. subspicatus</em>. However, protease treatment of erythrocytes showed no effect on <em>C. infusiformis</em> lectin activity, and decreased the lectin activity of <em>D. dimorphus</em>. Lectins of members of chlorophyceae have shown unique glycoprotein binding specificities as their lectin activity was specifically inhibited by glycoproteins exhibiting complex O-glycans, such as bovine submaxillary mucin, porcine stomach mucin and fetuin. All the algal cultures expressed maximum lectin activity during stationary phase of growth except <em>S. quadricauda</em> which expressed maximum lectin activity during mid-log to stationary phase of cultivation. Possibly, it is a new report on cell surface bound lectins from unexplored members of chlorophyceae for lectin activity. |
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| Date |
2018-07-09T09:58:23Z
2018-07-09T09:58:23Z 2018-07 |
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| Type |
Article
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| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/44683 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJEB Vol.56(07) [July 2018]
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