CSIR Central

ARL11 regulates lipopolysaccharide-stimulated macrophage activation by promoting mitogen-activated protein kinase (MAPK) signaling.

IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh


Field	Value

Title	ARL11 regulates lipopolysaccharide-stimulated macrophage activation by promoting mitogen-activated protein kinase (MAPK) signaling.

Creator	Arya, Subhash B Kumar, Gaurav Kaur, Harmeet Kaur, Amandeep Tuli, Amit

Subject	QR Microbiology

Description	DP-ibosylation factor-ike GTPase () is a cancer-predisposing gene that has remained functionally uncharacterized to date. In this study, we report that ARL11 is endogenously expressed in mouse and human macrophages and regulates their activation in response to lipopolysaccharide (LPS) stimulation. Accordingly, depletion of ARL11 impaired both LPS-stimulated pro-inflammatory cytokine production by macrophages and their ability to control intracellular replication of LPS-stimulated activation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) was substantially compromised in -silenced macrophages. In contrast, increased expression of ARL11 led to constitutive ERK1/2 phosphorylation, resulting in macrophage exhaustion. Finally, we found that ARL11 forms a complex with phospho-ERK in macrophages within minutes of LPS stimulation. Taken together, our findings establish ARL11 as a novel regulator of ERK signaling in macrophages, required for macrophage activation and immune function.

Publisher	ASBMB

Date	2018-06-22

Type	Article PeerReviewed

Relation	http://www.jbc.org/content/293/25/9892.long http://crdd.osdd.net/open/2105/

Identifier	Arya, Subhash B and Kumar, Gaurav and Kaur, Harmeet and Kaur, Amandeep and Tuli, Amit (2018) ARL11 regulates lipopolysaccharide-stimulated macrophage activation by promoting mitogen-activated protein kinase (MAPK) signaling. The Journal of biological chemistry, 293 (25). pp. 9892-9909. ISSN 1083-351X