Effect of bovine serum albumin immobilized Au-ZnO-graphene nanocomposite on human ovarian cancer cell
IR@CGCRI: CSIR-Central Glass and Ceramic Research Institute, Kolkata
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| Title |
Effect of bovine serum albumin immobilized Au-ZnO-graphene nanocomposite on human ovarian cancer cell
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| Creator |
Naskar, Atanu
Bera, Susanta Bhattacharya, Rahul Roy, Sib Sankar Jana, Sunirmal |
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| Subject |
Engineering Materials
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| Description |
Present work reports for the first time on successful in-situ synthesis of Au incorporated ZnO-graphene nanocomposites (AZG) by one pot surfactant free low temperature (similar to 95 degrees C) solution process. Zinc acetate dihydrate and graphene oxide with varying content of chloroauric acid (5 mM, 10 mM and 50 mM) were used as the precursor materials. The immobilization of bovine serum albumin (BSA) with a particular AZG2 nanocomposite (derived from the precursor medium with 10 mM chloroauric acid) was also made separately. X-ray diffraction, field emission scanning and transmission electron microscopic analyses confirmed the presence ZnO and Au nanoparticles (NPs), distributed uniformly in CCG matrix of the sample. The average particle size of ZnO and Au nanoparticle were similar to 11.3 nm and 30-60 nm, respectively as obtained from TEM images of AZG2 nanocomposite. FTIR, Raman, UV-visible and X-ray photoelectron spectroscopic characterizations also were carried out to confirm the existence of chemical interaction/complexation that happened between the available oxygen functional groups of CCG with the inorganic moieties (ZnO/Zn2+ and Au NPs) of AZG nanocomposites. An existence of interaction between BSA and AZG2 nanocomposite was confirmed by UV-Visible and photoluminescence spectral analyses. Initially, the in vitro cytotoxicity and quantitative cell viability (CV) of human ovarian teratocarcinoma cell line, PA1 was investigated to a maximum concentration of 200 mg/ml for ZO, ZG, AZG1, AZG2 and AZG3 sample. The CV was again repeated on BSA-AZG2 nanocomposite (as AZG2 shows maximum cell viability) and a maximum CV of the cancer cells was found (similar to 92% of the viable cells at 50 mu g/ml dose). This work could have fundamental importance in biomedical applications. (C) 2017 Elsevier B.V. All rights reserved.
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| Publisher |
Elsevier
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| Date |
2018-02
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://cgcri.csircentral.net/4327/1/atanu.pdf
Naskar, Atanu and Bera, Susanta and Bhattacharya, Rahul and Roy, Sib Sankar and Jana, Sunirmal (2018) Effect of bovine serum albumin immobilized Au-ZnO-graphene nanocomposite on human ovarian cancer cell. Journal of Alloys and Compounds, 734. pp. 66-74. ISSN 0925-8388 |
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| Relation |
http://cgcri.csircentral.net/4327/
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