Electrochemical Impedance Analysis of Biofunctionalized Conducting Polymer-Modified Graphene-CNTs Nanocomposite for Protein Detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Electrochemical Impedance Analysis of Biofunctionalized Conducting Polymer-Modified Graphene-CNTs Nanocomposite for Protein Detection
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| Creator |
Singal, Shobhita
Srivastava, Avanish K. -, Rajesh |
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| Subject |
Materials Science
Applied Physics/Condensed Matter Nanoscience/ Nanotechnology |
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| Description |
We report an electrodeposited poly(pyrrole-co-pyrrolepropylic acid) copolymer modified electroactive graphene-carbon nanotubes composite deposited on a glassy carbon electrode to detect the protein antigen (cTnI). The copolymer provides pendant carboxyl groups for the site-specific covalent immobilization of protein antibody, anti-troponin I. The hybrid nanocomposite was used as a transducer for biointerfacial impedance sensing for cTnI detection. The results show that the hybrid exhibits a pseudo capacitive behaviour with a maximum phase angle of 49 degrees near 1 Hz, which is due to the inhomogeneous and porous structure of the hybrid composition. The constant phase element of copolymer is 0.61 (n = 0.61), whereas, it is 0.88 (n = 0.88) for the hybrid composites, indicating a comparatively homogeneous microstructure after biomolecular functionalization. The transducer shows a linear change in charge transfer characteristic (R-et) on cTnI immunoreaction for spiked human serum in the concentration range of 1.0 pg mL(-1) - 10.0 ng mL(-1). The sensitivity of the transducer is 167.8 +/- 14.2 Omega cm(2) per decade, and it also exhibits high specificity and good reproducibility.
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| Publisher |
SpringerOpen
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| Date |
2017-06
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/2685/1/Electrochemical%20Impedance%20Analysis%20of%20Biofunctionalized%20Conducting%20Polymer-Modified%20Graphene-CNTs%20Nanocomposite%20for%20Protein%20Detection.pdf
Singal, Shobhita and Srivastava, Avanish K. and -, Rajesh (2017) Electrochemical Impedance Analysis of Biofunctionalized Conducting Polymer-Modified Graphene-CNTs Nanocomposite for Protein Detection. Nano-Micro Letters, 9 (1). pp. 1-9. ISSN 2311-6706 |
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| Relation |
http://npl.csircentral.net/2685/
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