Isolation and amplification of genomic DNA from nutmeg mace
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
Isolation and amplification of genomic DNA from nutmeg mace
|
|
| Creator |
Swetha, V P
Parvathy, V A Sheeja, T E Sasikumar, B |
|
| Subject |
Nutmeg
<i>Myristica fragrans</i> Recalcitrant Restriction digestion RAPD <em>rbcL</em> ITS |
|
| Description |
533-537
A reliable and efficient protocol for isolation and amplification of genomic DNA from dried mace of <em>Myristica fragrans</em>, was developed. The yield of genomic DNA was 231.4 µg g<sup>-1</sup> and 306.8 µg g<sup>-1</sup>, respectively for the samples procured from the farm and market. The absorbance ratio at A260/A280 was greater than 1.8 indicating the good quality of DNA. Complete restriction digestion and PCR amplification of genomic DNA further confirmed the quality of isolated DNA. |
|
| Date |
2018-10-23T11:52:02Z
2018-10-23T11:52:02Z 2018-07 |
|
| Type |
Article
|
|
| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/45270 |
|
| Language |
en_US
|
|
| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
|
|
| Publisher |
NISCAIR-CSIR, India
|
|
| Source |
IJBT Vol.17(3) [July 2018]
|
|