Electrochemical features of the interaction between (R)-/(S)-methyl-2- (5-fluorouracil-1-acetamide)-3-phenylpropionate and DNAs
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
Electrochemical features of the interaction between (R)-/(S)-methyl-2- (5-fluorouracil-1-acetamide)-3-phenylpropionate and DNAs
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| Creator |
Liu, Aili
Dong, Xiaomei Jin, Huile Wang, Shun Jiang, Weizhong |
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| Subject |
Cyclic voltammetry
DNA Gold electrode Intercalation (R)/(S)-5FUPPM |
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| Description |
398-403
Cyclic voltammetry was exploited to understand the interaction between chiral methyl-2-(5- fluorouracil-1-acetamide)-3-phenylpropionate molecules (denoted as (R)- or (S)-5FUPPM) and double-stranded (ds) or G-quadruplex (G4) DNAs. Using Fe(CN)<sub>6</sub><sup>3- </sup>as the redox mark, experiments illustrate that the anodic peak of Fe(CN)<sub>6</sub><sup>3- </sup>oxidation decreases as the concentration of 5FUPPM in the solution is increased. Calculation with the Langmuir equation yields the binding equilibrium constant of 9.1 × 10<sup>3 </sup>for the dsDNA and (S)-5FUPPM and of 3.3 × 10<sup>3</sup> for dsDNA and (R)-5FUPPM, suggesting that (S)-5FUPPM has more favorable interaction with dsDNA. On the other hand, the calculated binding constant between G4-DNA and (S)-5FUPPM is 1.6 × 10<sup>4</sup>, as opposed to 3.2 × 10<sup>4</sup> between G4-DNA and (R)-5FUPPM. The results suggest that the binding selectivity of (R)-5FUPPM with DNAs, defined with (<em>K</em><sub>G4-DNA</sub><em>/K</em><sub>dsDNA</sub>), is about 5 times larger than that of (S)-5FUPPM and DNAs, which is consistent with their inhibition rate on tumor cell. The information achieved here suggests that electrochemical characterization can provide useful information for the screening of new drug candidates. |
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| Date |
2018-11-29T05:45:54Z
2018-11-29T05:45:54Z 2018-12 |
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| Type |
Article
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| Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/45455 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBB Vol.55(6) [December 2018]
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