Three-dimensional cellulose-hydroxyapatite nanocomposite enriched with dexamethasone loaded metal-organic framework: a local drug delivery system for bone tissue engineering
IR@NML: CSIR-National Metallurgical Laboratory, Jamshedpur
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Title |
Three-dimensional cellulose-hydroxyapatite nanocomposite enriched with dexamethasone loaded metal-organic framework: a local drug delivery system for bone tissue engineering
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Creator |
Sarkar, C
Chowdhuri, A R Garai, S Chakraborty, J Sahu, S K |
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Subject |
Nano Composites
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Description |
Three-dimensional cellulose-hydroxyapatite nanocomposite integrated with dexamethasone loaded metal organic framework (HA/DMOF) has been synthesized as a local drug delivery system for bone tissue engineering. Initially, in situ dexamethasone encapsulated metal-organic frameworks (DMOFs) were developed and characterized. The synthesized DMOFs are 60-80nm in size with rhombohedral morphology. Results showed that nearly 16% dexamethasone (Dex) was loaded in DMOFs. These synthesized DMOF nanoparticles were efficiently integrated with carboxymethyl cellulose-hydroxyapatite nanocomposite for the development of three dimensional localized drug delivery system, namely, HA/DMOF. The synthesized HA/DMOF nanocomposite was structurally characterized using various techniques. The mechanical properties of HA/DMOFs were also measured by means of compression test. It was found that the compressive strength and compressive modulus of HA/DMOF nanocomposite are 16.31.57MPa and 0.54 +/- 0.073GPa respectively, which are in the range of cancellous bone. In-vitro release behaviour of Dex from both DMOFs and HA/DMOFs was examined in phosphate buffered solution. It was found that Dex molecules have been released very slowly from HA/DMOF nanocomposite compared to DMOF nanoparticles, and it was sustained for 4weeks. Cytocompatibility of HA/DMOF nanocomposite was evaluated against pre-osteoblast MC3T3 cells. It was found that the synthesized HA/DMOF nanocomposite is compatible to MC3T3 cells. Moreover, the ALP activity and extracellular mineralization capability of cells were enhanced when cultured with HA/DMOF nanocomposite. Results showed that the synthesized HA/DMOF nanocomposite is a promising material for possible therapeutic as well as load-bearing orthopedic applications.
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Publisher |
Elsevier
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Date |
2019-08
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Type |
Article
PeerReviewed |
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Relation |
https://doi.org/10.1007/s10570-019-02618-3
http://eprints.nmlindia.org/7965/ |
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Identifier |
Sarkar, C and Chowdhuri, A R and Garai, S and Chakraborty, J and Sahu, S K (2019) Three-dimensional cellulose-hydroxyapatite nanocomposite enriched with dexamethasone loaded metal-organic framework: a local drug delivery system for bone tissue engineering. Cellulose, 26(12) (IF-4.210). pp. 7253-7269.
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