Molecular dynamics simulation approach to explore atomistic molecular mechanism of peroxidase activity of apoptotic cytochrome c mutants
IR@CECRI: CSIR-Central Electrochemical Research Institute, Karaikudi
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| Title |
Molecular dynamics simulation approach to explore atomistic molecular mechanism of peroxidase activity of apoptotic cytochrome c mutants
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| Creator |
Muneeswaran, G.
Pandiaraj, M. Kartheeswaran, S. Sankaralingam, M. Muthukumar, K. Karunakaran, C. |
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| Subject |
Electrochemical Sensors
Electrodics and Electrocatalysis |
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| Description |
Mutations in cytochrome c (Cyt c) have been reported in tuning peroxidase activity, which in-turn cause Cyt c
release from mitochondria and early apoptosis. However, the molecular tuning mechanism underlying this activity remains elusive. Herein, multiple 20 ns molecular dynamics (MD) simulations of wild type (WT), Y67F and
K72W mutated Cyt c in aqueous solutions have been carried out to study how the changes in structural features
alters the peroxidase activity of the protein. MD simulation results indicate that Y67F mutation caused, (i)
increased distances between critical electron-transfer residues, (ii) higher fluctuations in omega loops, and (iii)
weakening of intraprotein hydrogen bonds result in open conformation at heme crevice loop in Cyt c leading to an
enhanced peroxidase activity. Interestingly, the aforementioned structural features are strengthened in K72W
compared to WT and Y67F, which triggers K72W mutated Cyt c into a poor peroxidase. Essential dynamics results
unveil that first two eigenvectors are responsible for overall motions of WT, Y67F and K72W mutated Cyt c. This
study thus provides atomic level insight into molecular mechanism of peroxidase activity of Cyt c, which will help in designing novel Cyt c structures that is more desirable than natural Cyt c for biomedical and industrial
processes
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| Publisher |
Elsevier
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| Date |
2018-04-14
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://cecri.csircentral.net/3179/1/302-2018.pdf
Muneeswaran, G. and Pandiaraj, M. and Kartheeswaran, S. and Sankaralingam, M. and Muthukumar, K. and Karunakaran, C. (2018) Molecular dynamics simulation approach to explore atomistic molecular mechanism of peroxidase activity of apoptotic cytochrome c mutants. Informatics in Medicine Unlocked, 11. pp. 51-60. ISSN 2352-9148 |
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| Relation |
http://www.elsevier.com/locate/imu
http://cecri.csircentral.net/3179/ |
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