(D)-p-Hydroxyphenylglycine production by thermostable D-hydantoinase from Brevibacillus parabrevis-PHG1
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
(D)-p-Hydroxyphenylglycine production by thermostable D-hydantoinase from Brevibacillus parabrevis-PHG1
|
|
| Creator |
Nandanwar, Hemraj
Prajapati, Rajnikant Hoondal, G S |
|
| Subject |
QR Microbiology
|
|
| Description |
his study was aimed at the investigation of D-hydantoinase from newly isolated strains of bacteria for overproduction of D-p-hydroxyphenylglycine. It was also hoped to develop a D-hydantoinase with suitable physicochemical parameters to make a successful process for other D-amino acids. D-hydantoinase was isolated from a Gram positive bacterial strain PHG1, identified as Brevibacillus parabrevis based on 16S rRNA gene sequence analysis. The strain showed hydantoinase activity of 5.0 U/ml of broth with hydantoin as substrate, at a cell turbidity of 4.8 at 600 nm. The enzyme was purified to homogeneity with native and subunit molecular mass of ≈154 kDa and ≈43 kDa, respectively. The specific activity of the enzyme was found to be ≈750 μmole/min/mg. D,L-p-hydroxyphenylhydantoin was found to be the preferred substrate after unsubstituted hydantoin. The optimum temperature and pH for enzyme activity were 70°C and 8.5, respectively, with a half-life of 120 min at 70°C. Supplementing with 0.32 mM Mn2+ in the growth medium resulted in ≈3-fold increase in enzyme activity. Ninety-five percent conversion efficiency of D-hydantoinase for D,L-p-hydroxyphenylhydantoin (10% w/v) into N-carbamoyl-(D)-p-hydroxyphenylglycine, better pH-temperature stability and broad substrate specificity signify the usefulness of this enzyme for production of D-p-hydroxyphenylglycine and other D-amino acids of industrial importance.
|
|
| Publisher |
Taylor and francis group
|
|
| Date |
2013-01-18
|
|
| Type |
Article
PeerReviewed |
|
| Relation |
https://www.tandfonline.com/doi/abs/10.3109/10242422.2012.755962
http://crdd.osdd.net/open/2428/ |
|
| Identifier |
Nandanwar, Hemraj and Prajapati, Rajnikant and Hoondal, G S (2013) (D)-p-Hydroxyphenylglycine production by thermostable D-hydantoinase from Brevibacillus parabrevis-PHG1. Biocatalysis and Biotransformation, 31 (1). ISSN 1024-2422
|
|