NorA efflux pump inhibitory activity of coumarins from Mesua ferrea.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
NorA efflux pump inhibitory activity of coumarins from Mesua ferrea.
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| Creator |
Roy, Somendu K
Kumari, Neela Pahwa, Sonika Agrahari, Udai C Bhutani, Kamlesh K Jachak, Sanjay M Nandanwar, Hemraj |
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| Subject |
QR Microbiology
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| Description |
The purpose of this investigation was to study the modulator and efflux pump inhibitor activity of coumarins isolated from Mesua ferrea against clinical strains as well as NorA-over expressed strain of Staphylococcus aureus 1199B. Seven coumarins were tested for modulator activity using ethidium bromide (EtBr) as a substrate. Compounds 1, 4-7 modulated the MIC of EtBr by ≥ 2 fold against wild type clinical strains of S. aureus 1199 and S. aureus 1199B, whereas compounds 4-7 modulated the MIC of EtBr by ≥ 16 fold against MRSA 831. Compounds 1, 4-7 also reduced the MIC of norfloxacin by ≥ 8 fold against S. aureus 1199B, and 4-6 reduced the MIC of norfloxacin by ≥ 8 fold against MRSA 831 at half of their MICs. Inhibition of EtBr efflux by NorA-overproducing S. aureus 1199B and MRSA 831 confirmed the role of compounds 4-6 as NorA efflux pump inhibitors (EPI). Dose-dependent activity at sub-inhibitory concentration (6.25 μg/mL) suggested that compounds 4 and 5 are promising EPI compared to verapamil against 1199B and MRSA 831 strains.
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| Publisher |
Elsevier Science
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| Date |
2013-10
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| Type |
Article
PeerReviewed |
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| Relation |
https://linkinghub.elsevier.com/retrieve/pii/S0367-326X(13)00186-X
http://crdd.osdd.net/open/2452/ |
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| Identifier |
Roy, Somendu K and Kumari, Neela and Pahwa, Sonika and Agrahari, Udai C and Bhutani, Kamlesh K and Jachak, Sanjay M and Nandanwar, Hemraj (2013) NorA efflux pump inhibitory activity of coumarins from Mesua ferrea. Fitoterapia, 90. pp. 140-50. ISSN 1873-6971
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