An electrochemical biosensor based on novel butylamine capped CZTS nanoparticles immobilized by uricase for uric acid detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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| Title |
An electrochemical biosensor based on novel butylamine capped CZTS nanoparticles immobilized by uricase for uric acid detection
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| Creator |
Jain, Shefali
Verma, Shilpi Singh, Surinder P. Sharma, Shailesh Narain |
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| Subject |
Biophysics
Biotechnology & Applied Microbiology Analytical Chemistry Electrochemistry Nanoscience/ Nanotechnology |
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| Description |
Quaternary chalcopyrite, i.e., Cu(2)ZuSnS(4) (CZTS) nanopartides films have been proposed as a novel matrix system for enzyme-based electrochemical biosensors providing a non-toxic, low-cost alternative for the fabrication of bioelectrodes. The easy tuneability of the band gap of CZTS by varying the cation ratio and size of nanoparticles facilitate to impart desirable electrical properties in the material. Butylamine capped spherical CZTS nanoparticles of size 15-16 nm and band gap 2.65 eV have been synthesized by colloidal hot injection technique. The films of CZTS onto ITO substrates are deposited using dip coating technique, and uricase enzyme have been immobilized onto CZTS films using EDC-NHS binding chemistry. Electrochemical analyses of this bioelectrode revealed that the uricase/CZTS/ITO/glass electrode exhibits good linearity over a wide range of 0-700 M uric acid concentration with a limit of detection (LOD) of 0.066 M. The low value of 0.13 x 10(-4) M of Michaelis-Menten constant (Km) indicate the enhanced affinity of inunobilized enzyme (uricase) towards uric acid. Thus, the present report confirms the promising application of the p-type CZTS thin film matrix for the realization of an electrochemical biosensor.
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| Publisher |
Elsevier
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| Date |
2019-02-15
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/4104/1/An%20electrochemical%20biosensor.pdf
Jain, Shefali and Verma, Shilpi and Singh, Surinder P. and Sharma, Shailesh Narain (2019) An electrochemical biosensor based on novel butylamine capped CZTS nanoparticles immobilized by uricase for uric acid detection. Biosensors and Bioelectronics, 127. pp. 135-141. ISSN 0956-5663 |
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| Relation |
http://npl.csircentral.net/4104/
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