Highly Luminescent-Paramagnetic Nanophosphor Probes for In Vitro High-Contrast Imaging of Human Breast Cancer Cells
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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| Title |
Highly Luminescent-Paramagnetic Nanophosphor Probes for In Vitro High-Contrast Imaging of Human Breast Cancer Cells
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| Creator |
Gupta, Bipin Kumar
Narayanan, Tharangattu N. Vithayathil, Sajna Antony Lee, Yean Koshy, Shyny Reddy, Arava Leela Mohana Saha, Avishek Shanker, V. Singh, V. N. Kaipparettu, Benny Abraham Marti, Angel A. Ajayan, Pulickel M. |
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| Subject |
Chemistry
Materials Science Applied Physics/Condensed Matter Nanoscience/ Nanotechnology Physical Chemistry/Chemical Physics |
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| Description |
Highly luminescentparamagnetic nanophosphors have a seminal role in biotechnology and biomedical research due to their potential applications in biolabeling, bioimaging, and drug delivery. Herein, the synthesis of high-quality, ultrafine, europium-doped yttrium oxide nanophosphors (Y1.9O3:Eu0.13+) using a modified solgel technique is reported and in vitro fluorescence imaging studies are demonstrated in human breast cancer cells. These highly luminescent nanophosphors with an average particle size of 6 nm provide high-contrast optical imaging and decreased light scattering. In vitro cellular uptake is shown by fluorescence microscopy, which visualizes the characteristic intense hypersensitive red emission of Eu3+ peaking at 610 nm (5D07F2) upon 246 nm UV light excitation. No apparent cytotoxicity is observed. Subsequently, time-resolved emission spectroscopy and SQUID magnetometry measurements demonstrate a photoluminescence decay time in milliseconds and paramagnetic behavior, which assure applications of the nanophosphors in biomedical studies.
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| Publisher |
Wiley
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| Date |
2012-10-08
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/3545/1/Highly%20Luminescent%E2%80%93Paramagnetic.pdf
Gupta, Bipin Kumar and Narayanan, Tharangattu N. and Vithayathil, Sajna Antony and Lee, Yean and Koshy, Shyny and Reddy, Arava Leela Mohana and Saha, Avishek and Shanker, V. and Singh, V. N. and Kaipparettu, Benny Abraham and Marti, Angel A. and Ajayan, Pulickel M. (2012) Highly Luminescent-Paramagnetic Nanophosphor Probes for In Vitro High-Contrast Imaging of Human Breast Cancer Cells. Small, 8 (19). pp. 3028-3034. ISSN 1613-6810 |
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| Relation |
http://npl.csircentral.net/3545/
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