A highly efficient rare earth metal oxide nanorods based platform for aflatoxin detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive InfoField | Value | |
Title |
A highly efficient rare earth metal oxide nanorods based platform for aflatoxin detection
|
|
Creator |
Singh, Jay
Roychoudhury, Appan Srivastava, Manish Solanki, Pratima R. Lee, Dong Won Lee, Seung Hee Malhotra, B. D. |
|
Subject |
Materials Science
|
|
Description |
The nanostructured rare earth metal oxide (samarium oxide, n-Sm2O3) nanorods, prepared using a forced hydrolysis technique, have been electrophoretically deposited (EPD) onto an indium-tin-oxide (ITO) glass substrate. This novel platform has been utilized for co-immobilization of monoclonal antibodies of aflatoxin B-1 (Ab-AFB(1)) and bovine serum albumin (BSA) via electrostatic interactions for food toxin (AFB(1)) detection. Thus prepared n-Sm2O3 nanorods have been characterized by X-ray diffraction (XRD), atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopic techniques. The results of electrochemical response studies of the BSA/Ab-AFB(1)/n-Sm2O3/ITO immunoelectrode obtained as a function of aflatoxin concentration reveal a linearity of 10-700 pg mL(-1), a detection limit of 57.82 pg mL(-1) cm(-2), a response time of 5 s and a sensitivity of 48.39 mu A pg(-1) mL(-1) cm(-2) with a regression coefficient of 0.961. The association constant (K-a) for antigen-antibody interactions obtained is 47.9 pg mL(-1), which indicates high affinity of antibodies towards the antigen (AFB1). The application of n-Sm2O3 modified electrode for immunosensor analysis offers a novel platform and efficient strategy for the application of rare earth metal oxide materials in bioelectronics.
|
|
Publisher |
Royal Society of Chemistry
|
|
Date |
2013
|
|
Type |
Article
PeerReviewed |
|
Format |
application/pdf
|
|
Identifier |
http://npl.csircentral.net/2967/1/3.pdf
Singh, Jay and Roychoudhury, Appan and Srivastava, Manish and Solanki, Pratima R. and Lee, Dong Won and Lee, Seung Hee and Malhotra, B. D. (2013) A highly efficient rare earth metal oxide nanorods based platform for aflatoxin detection. Journal of Materials Chemistry B, 1. pp. 4493-4503. ISSN 2050-750X |
|
Relation |
http://npl.csircentral.net/2967/
|
|