Quantum Dots Self Assembly Based Interface for Blood Cancer Detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Quantum Dots Self Assembly Based Interface for Blood Cancer Detection
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| Creator |
Sharma, Aditya
Sumana, Gajjala Sapra, Sameer Malhotra, Bansi Dhar |
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| Subject |
Chemistry
Physical Chemistry Materials Science |
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| Description |
Results of the studies related to fabrication of, sensitive electrochemical biosensor using an interface based On quantum dots (QDs) self-assembly is reported. The QDs assembly is sought to provide improved fundamental characteristics to the electrode interface in terms of electro-active surface area, diffusion coefficient, and electron, transfer kinetics. This QDs modified electrode has been utilized to serve as a transducer surface for covalent immobilization of chronic myelogenous leukemia (CML) specific probe oligonucleotide, designed from the BCR-ABL fusion gene. The electrochemical characteristics of this biosensor toward various designed synthetic oligonucleotides reveal a significant enhancement in its mismatch discrimination capability compared to the biosensing assay without QDs under similar experimental conditions. The sensing characteristics of this biosensor Offer a potential for detection of target oligonucleotide at a concentration as low as 1.0 pM. Furthermore, the PCR-amplified CML-positive patient samples with various BCR-ABL. transcript ratios can be electrochemically distinguished from healthy samples, indicating promising application of the QDs based biosensor for clinical investigations.
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| Publisher |
American Chemical Society
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| Date |
2013-07-09
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/3084/1/Quantum%20Dots%20Self%20Assembly.pdf
Sharma, Aditya and Sumana, Gajjala and Sapra, Sameer and Malhotra, Bansi Dhar (2013) Quantum Dots Self Assembly Based Interface for Blood Cancer Detection. LANGMUIR, 29. 8753-8762. ISSN 0743-7463 |
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| Relation |
http://npl.csircentral.net/3084/
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