Nanostructured magnesium oxide biosensing platform for cholera detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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| Title |
Nanostructured magnesium oxide biosensing platform for cholera detection
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| Creator |
Patel, Manoj K.
Ali, Md. Azahar Agrawal, Ved V. Ansari, Z. A. Ansari, S. G. Malhotra, B. D. |
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| Subject |
Applied Physics/Condensed Matter
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| Description |
We report fabrication of highly crystalline nanostructured magnesium oxide (NanoMgO, size >30 nm) film electrophoretically deposited onto indium-tin-oxide (ITO) glass substrate for Vibrio cholerae detection. The single stranded deoxyribonucleic acid (ssDNA) probe, consisting of 23 bases (O1 gene sequence) immobilized onto NanoMgO/ITO electrode surface, has been characterized using electrochemical, Fourier Transform-Infra Red, and UltraViolet-visible spectroscopic techniques. The hybridization studies of ssDNA/NanoMgO/ITO bioelectrode with fragmented target DNA conducted using differential pulse voltammetry reveal sensitivity as 16.80 nA/ng/cm(2), response time of 3 s, linearity as 100-500 ng/mu L, and stability of about 120 days.
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| Publisher |
American Institute of Physics
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| Date |
2013-04-08
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/3112/1/Nanostructured%20magnesium%20oxide%20biosensing%20platform%20for%20cholera.pdf
Patel, Manoj K. and Ali, Md. Azahar and Agrawal, Ved V. and Ansari, Z. A. and Ansari, S. G. and Malhotra, B. D. (2013) Nanostructured magnesium oxide biosensing platform for cholera detection. Applied Physics Letters, 102 (14). 144106-1-144106-5. ISSN 0003-6951 |
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| Relation |
http://npl.csircentral.net/3112/
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