A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM
|
|
| Creator |
Sharma, Sandeep
Muddassir, Mohd Muthusamy, Saraladevi Vaishnav, Pardeep Kumar Singh, Manish Sharma, Deepak Kanagarajan, Selvaraju Shanmugam, Vijayakumar |
|
| Subject |
QR Microbiology
|
|
| Description |
Classical plant uptake is limited to hydrophilic or water-dispersible material. Therefore, in order to test the uptake behaviour of hydrophobic particles, here, we tested the fate of hydrophobic particles (oleylamine coated Cu2-xSe NPs (CS@OA)) in comparison to hydrophilic particles (chitosan-coated Cu2-xSe NPs (CS@CH)) by treatment on the plant roots. Surprisingly, hydrophobic CS@OA NPs have been found to be similar to 1.3 times more efficient than hydrophilic CS@CH NPs in tomato plant root penetration. An atomic force microscopy (AFM) adhesion force experiment confirms that hydrophobic NPs experience non-spontaneous yet energetically favorable root trapping and penetration. Further, a relative difference in the hydrophobic vs. hydrophilic NPs movement from roots to shoots has been observed and found related to the change in protein corona as identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) analysis. Finally, the toxicity assays at the give concentration showed that Cu2-xSe NPs lead to non-significant toxicity as compared to control. This technology may find an advantage in fertilizer application.
|
|
| Publisher |
Nature publishing group
|
|
| Date |
2020-11-06
|
|
| Type |
Article
PeerReviewed |
|
| Relation |
https://www.nature.com/articles/s41598-020-75685-3
http://crdd.osdd.net/open/2628/ |
|
| Identifier |
Sharma, Sandeep and Muddassir, Mohd and Muthusamy, Saraladevi and Vaishnav, Pardeep Kumar and Singh, Manish and Sharma, Deepak and Kanagarajan, Selvaraju and Shanmugam, Vijayakumar (2020) A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM. Scientific reports, 10 (1). ISSN 2045-2322
|
|