Ethylenediamine assisted functionalization of self-organized poly (D, L-lactide-co-glycolide) patterned surface to enhance cancer cell isolation
IR@CMERI: CSIR- Central Mechanical Engineering Research Institute (CMERI), Durgapur
View Archive Info| Field | Value | |
| Title |
Ethylenediamine assisted functionalization of self-organized poly (D, L-lactide-co-glycolide) patterned surface to enhance cancer cell isolation
|
|
| Creator |
Jaiswal, Namita
Hens, Abhiram Chatterjee, Manosree Mahata, Nibedita Chanda, Nripen |
|
| Subject |
Chemistry
|
|
| Description |
Abstract Protein functionalized micro-scale patterned structures are developed using a biocompatible polymer PLGA (poly (D, L-lactide-co-glycolide)) via thin film dewetting and by step-wise chemical conjugations with EDA (ethylenediamine) and anti-EpCAM (Epithelial Cell Adhesion olecule) antibodies to target the epithelial cell adhesion molecules of cancer cells. The effectiveness of such protein functionalized patterned surface is checked through cell isolation process using blood samples spiked with different cancer cells such as MCF-7, A549, MDA-MB-231. An efficient capture yield of 92% is obtained with MCF-7 cells over a two hour incubation time. The study demonstrates the effects of cell concentration and incubation time on the binding of cancer cells to the modified patterned surfaces. For the first time, a simple and inexpensive method is reported to fabricate functionalized PLGA patterned surface for an efficient isolation of cancer cells from diluted blood samples. The method shows the potential to be used as an effective platform for the development of an improved circulating tumor cell (CTC) isolation device from the clinical blood sample.
|
|
| Publisher |
Elsevier
|
|
| Date |
2019-01-15
|
|
| Type |
Article
PeerReviewed |
|
| Identifier |
Jaiswal, Namita and Hens, Abhiram and Chatterjee, Manosree and Mahata, Nibedita and Chanda, Nripen (2019) Ethylenediamine assisted functionalization of self-organized poly (D, L-lactide-co-glycolide) patterned surface to enhance cancer cell isolation. Journal of Colloid and Interface Science, 534. pp. 122-130.
|
|
| Relation |
http://cmeri.csircentral.net/624/
|
|