Development and validation of a novel RP-HPLC method for the simultaneous quantification of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol in a polyherbal formulation
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
Development and validation of a novel RP-HPLC method for the simultaneous quantification of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol in a polyherbal formulation
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| Creator |
Kshirsagar, Rishikesh Rajendra
Vaidya, Shivani Atul Jain, Vandana |
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| Subject |
ICH
Phytoconstituents Polyherbal formulation RP-HPLC Validation |
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| Description |
307-311
A novel, accurate, precise, and linear RP-HPLC method for the simultaneous estimation of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol in a polyherbal formulation was developed and validated as per ICH guidelines. A good chromatographic separation was achieved using gradient profile with the help of Shim-pack RP-HPLC column (250 mm*4.6 mm, 5 µm) and a mobile phase consisting of acetonitrile and 1% acetic acid was used. The column temperature was maintained at 28 °C throughout the run with a wavelength of 272 nm using UV-Visible detector. The retention time of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol was found to be 3.9, 6.7, 21.5, 44.6, and 45.9 min respectively. The linearity of ascorbic acid, gallic acid, ferulic acid, piperine, and thymol was found to be in a range of 10-100, 5-50, 1-10, 5-50, and 2-20 µg/mL, respectively with correlation coefficient >0.99. The high recovery values (98-102%) indicate satisfactory accuracy. The % RSD values were found to be less than 2% in the precision study which reveals that the method is precise. Hence the developed method can be used for quality control and quantitative analysis of extracts and commercial containing these selected phytoconstituents. |
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| Date |
2021-02-04T09:09:00Z
2021-02-04T09:09:00Z 2020-12 |
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| Type |
Article
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| Identifier |
0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscair.res.in/handle/123456789/56141 |
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| Language |
en_US
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| Relation |
Int. cl. (2015.01)- A61K 36/00
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJNPR Vol.11(4) [December 2020]
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