In vivo genomic expression of luteinizing hormone receptors and steroidogenic-enzyme in follicles of crossbred cows
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Title |
In vivo genomic expression of luteinizing hormone receptors and steroidogenic-enzyme in follicles of crossbred cows
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| Creator |
Satheshkumar, S
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| Subject |
Ovarian granulosa cell
LH receptor Steroidogenic enzyme Indian crossbred cows |
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| Description |
206-210
The present work was aimed at documenting the <em>in vivo</em> expression patterns of luteinizing hormone receptor (LHr) and cytochrome P450 aromatase enzyme (CYP19) genes in the granulosa cells (GC) of dominant follicles (DF) in Indian crossbred cows. Six crossbred cows were utilized for the study. Ultrasound guided <em>in vivo</em> follicular aspiration was performed on the specific days of the cycle viz., Day 0-oestrus (preovulatory follicle-POF); Day 6-wave I growing DF (GDF) and Day 8-wave I regressing DF (RDF). Twelve follicles in each category were aspirated and GCs were separated. Qualitative and semi-quantitative analysis of LHr and CYP-19 genes in GCs of all the three categories of the DFs were studied by conventional polymerase chain reaction (PCR) and densitometry analysis. PCR studies showed that mRNA expression for the LHr was enhanced in GDF comparable with that of POF, but significantly reduced in RDF. Analysis of relative expression levels revealed that the LHr expression was reduced by 1.5 folds in Day 8 RDF when compared to GDF. The expression levels of CYP19 gene in POF were greater than GDF and RDF respectively. Increased expression levels of these genes explained the ovulatory nature of POF when compared to wave I GDF. |
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| Date |
2021-02-12T04:52:53Z
2021-02-12T04:52:53Z 2020-07 |
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| Type |
Article
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| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/56220 |
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| Language |
en_US
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBT Vol.19(3) [July 2020]
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