Highly Efficient Bienzyme Functionalized Biocompatible Nanostructured Nickel Ferrite-Chitosan Nanocomposite Platform for Biomedical Application
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Highly Efficient Bienzyme Functionalized Biocompatible Nanostructured Nickel Ferrite-Chitosan Nanocomposite Platform for Biomedical Application
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| Creator |
Singh, Jay
Roychoudhury, Appan Srivastava,, Manish Chaudhary, Vidhi Prasanna, Radha Lee, Dong Won Lee, Seung Hee Malhotra, B. D. |
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| Subject |
Physical Chemistry
Materials Science Nanoscience/ Nanotechnology |
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| Description |
A new hybrid nanocomposite based on hydrothermally synthesized nanostructured NiFe2O4 (n-NiFe2O4) and chitosan (CH) has been explored for bienzyme (cholesterol esterase (ChEt) and cholesterol oxidase (ChOx)) immobilization for application as total cholesterol biosensor. Results of X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), Raman spectroscopy (RS) and vibrating sample magnetometer (VSM) studies demonstrate that the ChEt ChOx/n-NiFe2O4 CH composite film is successfully synthesized. The obtained ChEt ChOx/n-NiFe2O4 CH nanocomposite film shows large specific area, high conductivity, good biocompatibility, fast redox properties and improved antimicrobial activity. The fabricated ChEt ChOx/n-NiFe2O4 CH/ITO bioelectrode exhibits largely improved amperometric biosensing performance, i.e., good linearity (5-400 mg/dL), low detection limit (24.46 mg/dL cm(-2)), high sensitivity of 1.73 mu A/(mg/dL cm(-2)), fast response time of 15s, reproducibility of more than 15 times, shelf life of about 90 days and low Michaelis-Menten constant (K-m) value as 7.05 mg/dL (0.1825 mM). Furthermore, this modified bioelectrocle has been utilized for estimation of total cholesterol in human serum samples. This efficient strategy provides new insight into the design of novel flexible electrodes for a wide range of applications in biosensing, bioelectronics, and clinical applications.
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| Publisher |
American Chemical Society
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| Date |
2013-04-25
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/3189/1/Highly%20Efficient%20Bienzyme%20Functionalized.pdf
Singh, Jay and Roychoudhury, Appan and Srivastava,, Manish and Chaudhary, Vidhi and Prasanna, Radha and Lee, Dong Won and Lee, Seung Hee and Malhotra, B. D. (2013) Highly Efficient Bienzyme Functionalized Biocompatible Nanostructured Nickel Ferrite-Chitosan Nanocomposite Platform for Biomedical Application. Journal of Physical Chemistry C, 117 (16). pp. 8491-8502. ISSN 1932-7447 |
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| Relation |
http://npl.csircentral.net/3189/
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