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<b style="">Process optimization for the extraction of hyperforin and hypericin from St. John’s Wort </b><b style=""></b>

IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)


Field	Value

Creator	Koul, V K Koul, Suman

Date	2010-04-08T05:02:13Z 2010-04-08T05:02:13Z 2007-08

Identifier	0975-1092 (Online); 0972-592X (Print) http://hdl.handle.net/123456789/7873

Description	293-296 St. John’s Wort (<b><i style="">Hypericum perforatum</i> Linn.</b>), native to Europe and South America, grows wild in temperate and central parts of India. It has been used as a medicinal plant since ancient times for ulceration of kidneys, jaundice, gout and rheumatism. The antidepressant activity of St. John’s Wort extract has sparked great interest in the chemistry and biochemistry of its constituents, hyperforin and hypericin. The process described in this paper outlines the extraction of hyperforin by solvent hexane and thereafter extraction of hypericin from marc by commercial ethanol. The process parameters optimized for both the extractions are: solvent/plant material ratio, temperature, number of extractions and time for each extraction. Optimization was carried out by performing different sets of experiments (on 15kg scale) and varying one parameter at a time for each set of experiment. Depending on quality of plant material, under optimum conditions (i) Hexane extraction yielded 4 to 5% residue containing up to 38% Hyperforin, and (ii) Ethanol extraction yielded 20 to 22% residue containing up to 2.5% hypericin.

Language	en_US

Publisher	CSIR

Relation	<b>Int. cl.<sup>8</sup> </b><b>— </b><b></b>A61K 36/00, A61K 31/00, A61P 25/24

Source	NPR Vol.6(4) [July-August 2007]

Subject	<i style="">Hypericum perforatum</i> St. John’s Wort Hyperforin Hypericin Extraction Optimization

Title	<b style="">Process optimization for the extraction of hyperforin and hypericin from St. John’s Wort </b><b style=""></b>

Type	Article