Effect of carbon source concentration and culture duration on retreivability of bacteria from certain estuarine, coastal and offshore areas around the peninsular India.
IR@NIO: CSIR-National Institute Of Oceanography, Goa
View Archive Info| Field | Value | |
| Creator |
Goltekar, R.C.
Krishnan, K.P. DeSouza, M.J.B.D. Paropkari, A.L. LokaBharathi, P.A. |
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| Date |
2006-05-01T09:33:33Z
2006-05-01T09:33:33Z 2006 |
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| Identifier |
Current Science, vol.90(1), 103-106p.
http://drs.nio.org/drs/handle/2264/67 |
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| Description |
Improved culture-based approaches efficiently compliment the limitations encountered in molecular methods to delineate the diversity and functions of uncultured organisms. The present study endeavours to reduce the anomaly between total counts (TC) and retrievable plate counts as colony forming units (CFU). Retrievability was improved by decreasing nutrient concentration in the culture medium. Maximum retrievability of 10^4 CFU ml^–1 was obtained on 0.1% nutrient strength (100% corresponds to 8 g l^–1 nutrient broth with 1.5% agar-agar) with samples from the Bay of Bengal offshore and coastal waters. However, in the Arabian Sea, retrievability was maximum (10^2 CFU ml^–1) on 33 and 100% nutrient strength. By varying the nutrient concentration, retrievability could be enhanced to 24% of TC in the estuaries, 3–14% in coastal waters and 5% in offshore waters. Bacteria from relatively more dynamic estuarine systems seemed less resilient compared to the coastal and offshore populations, as they were best retrieved (105 CFU ml^–1) only on 1% nutrient strength.
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| Format |
64992 bytes
application/pdf |
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| Language |
en
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| Publisher |
Indian Academy of Sciences
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| Subject |
Bacterial retrievability
coastal and offshore colony forming units estuaries nutrient strength |
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| Title |
Effect of carbon source concentration and culture duration on retreivability of bacteria from certain estuarine, coastal and offshore areas around the peninsular India.
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| Type |
Article
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