Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Gupta, S
Singh, K Bhattacharya, A |
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| Subject |
Biochemistry & Molecular Biology
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| Description |
We prepared Polysulfone (PS) globules by pouring the PS solution droplets in to the non-solvent water. PS solutions (10, 15, and 20% (w/v), concentrations) of different viscosities resulted different physical appearances in globules The lipase immobilizing amount (4.32 mg/g) was maximum for PS 20% globules as it possessed maximum BET pore surface area. The olive oil hydrolytic reaction parameters were fitted into the Lineweaver-Burk plot The K(m) (apparent) values of all the immobilized globules were higher (83 3 mM) with respect to free lipases (62 5 mM) where as V(max) (apparent) values followed the reverse trend (129 8 U/mg for 20% and 120 5 U/mg for both 15% and 10% PS immobilized globules and 153 8 U/mg for the free lipase respectively) The hydrolytic activity to olive oil was decreased (similar to 16-20%) after five cycles. (C) 2010 Elsevier B.V. All rights reserved
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| Publisher |
ELSEVIER SCIENCE BVAMSTERDAMPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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| Date |
2011-09-20T12:03:18Z
2011-09-20T12:03:18Z 2010 |
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| Type |
Article
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| Identifier |
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
0141-8130 http://hdl.handle.net/123456789/12264 |
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| Language |
English
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