Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Singh, A
Reddy, MP Chikara, J Singh, S |
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| Subject |
Agriculture
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| Description |
A simple, rapid and cost effective protocol has been developed for high frequency regeneration using stem segments of elite genotypes (CSMCRI-I, CSMCRI-II and CSMCRI-III) of Jatropha curcas. Shoot bud induction (10-15 buds per explant) was achieved on Murashige and Skoog's (MS) medium supplemented with 1.0ml(-1) benzylaminopurine (BAP) in combination with 1.0mgl(-1) 6-furfurylamino purine (KN). Stem explant of CSMCRI-II showed highest response (65.3%) followed by CSMCRI-I and CSMCRI-III. These shoot buds developed into shoots when subcultured on MS medium supplemented with 0.5 mg l(-1) BAP and 1.0 mg l(-1) IAA (indole-3-acetic acid). Shoots of 4.0-5.0 cm length were harvested and cultured on MS medium containing different concentrations of indole-3-butyric acid (IBA) and 40% rooting was achieved in 0.1 mgl(-1) IBA after 5 weeks in all the genotypes used. For direct rooting, shoots of 4.0-5.0cm length were used and rooting was achieved by dipping the base of shoots in MS medium supplemented with 0.1 mg l(-1) IBA and 3.5% sodium alginate matrix and subsequently dropping in polymerization medium containing 2.0% calcium chloride. Encapsulated shoots were transferred in polybags filled with sterile soil wetted with sterile distilled water containing 0.5% broad-spectrum fungicide (Bavistine). Rooting could be achieved in 62% of shoots within 3 weeks. Rooted plantlets were successfully hardened and transferred to green house with 92% establishment. (C) 2009 Elsevier B.V. All rights reserved.
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| Publisher |
ELSEVIER SCIENCE BVAMSTERDAMPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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| Date |
2011-09-20T12:03:21Z
2011-09-20T12:03:21Z 2010 |
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| Type |
Article
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| Identifier |
INDUSTRIAL CROPS AND PRODUCTS
0926-6690 http://hdl.handle.net/123456789/12292 |
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| Language |
English
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