Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Balasubramanian, C
Ojha, RP Maiti, S Desideri, A |
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| Subject |
Chemistry
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| Description |
siRNA and microRNA (miRNA) are two classes of noncoding RNAs that carry out post-transcriptional gene regulation by interacting with the target mRNA. The structural features of siRNA/miRNA-mRNA complex play a crucial role in gene silencing. Here we have used computer modeling and simulation approach to (i) elucidate the possible structures of the partially complementary lin-4:lin-14 miRNA-mRNA complex and (ii) compare the structural features with the fully complementary lin-4:lin-14 siRNA-mRNA complex. The fully complementary siRNA complex maintains a canonical helical shape while the presence of mismatch base pairs and bulge induce a kinked structure in the miRNA complex. Docking of the simulated duplex models on to the argonaute protein gives a direct indication that in the siRNA-mRNA structure, the scissile phosphate of the mRNA strand is more accessible to the catalytic site as compared to the miRNA-mRNA structures, providing an explanation for the less prevalence of the cleavage in the later case.
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| Publisher |
AMER CHEMICAL SOCWASHINGTON1155 16TH ST, NW, WASHINGTON, DC 20036 USA
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| Date |
2011-09-20T12:07:04Z
2011-09-20T12:07:04Z 2010 |
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| Type |
Article
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| Identifier |
JOURNAL OF PHYSICAL CHEMISTRY B
1520-6106 http://hdl.handle.net/123456789/13083 |
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| Language |
English
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