Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Singh, A
Chauhan, NS Thulasiram, HV Taneja, V Sharma, R |
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| Subject |
Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels
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| Description |
Oxygenases are useful for the production of many industrially important molecules. Screening of an effluent treatment plant (ETP) sludge metagenomic library identified two clones encoding proteins, B1 and B2, with similarity to putative Flavin monooxygenases from Mesorhizobium loti and Sphingomonas wittichi, respectively. The deduced amino acid sequences show only 20% identity, but both have a paired Rossman fold and a flavin monooxygenase (FMO) motif. B1 and B2 appear to be members of the Flavin-containing monooxygenase and the Baeyer-Villiger monooxygenases subfamilies, respectively. When expressed in Escherichia coil, the two clones produced activities that oxidized indole to a mixture of indigo and indirubin pigments. These results suggest that B1 and B2 have potential as a biocatalyst in indigo/indirubin production. (C) 2010 Elsevier Ltd. All rights reserved.
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| Publisher |
ELSEVIER SCI LTDOXFORDTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
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| Date |
2011-09-20T12:07:05Z
2011-09-20T12:07:05Z 2010 |
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| Type |
Article
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| Identifier |
BIORESOURCE TECHNOLOGY
0960-8524 http://hdl.handle.net/123456789/13093 |
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| Language |
English
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